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Literature summary extracted from

  • Cai, L.; Zhao, X.; Jiang, T.; Qiu, J.; Owusu, L.; Ma, Y.; Wang, B.; Xin, Y.
    Prokaryotic Expression, identification and bioinformatics analysis of the Mycobacterium tuberculosis Rv3807c gene encoding the putative enzyme committed to decaprenylphosphoryl-D-arabinose synthesis (2014), Indian J. Microbiol., 54, 46-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.111 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.111 cytoplasm
-
 Mycobacterium tuberculosis 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis H37Rv
-
 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
3.1.3.111 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + H2O Mycobacterium tuberculosis
-
 trans,octacis-decaprenylphospho-beta-D-ribofuranose + phosphate
-
 ?
3.1.3.111 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 trans,octacis-decaprenylphospho-beta-D-ribofuranose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.45 Mycobacterium tuberculosis P9WFR5
-
-
2.4.2.45 Mycobacterium tuberculosis H37Rv P9WFR5
-
-
3.1.3.111 Mycobacterium tuberculosis P9WI53
-
-
3.1.3.111 Mycobacterium tuberculosis H37Rv P9WI53
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.111 Ni-NTA affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
2.4.2.45 trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis H37Rv trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
-
 ?
3.1.3.111 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + H2O
-
 Mycobacterium tuberculosis trans,octacis-decaprenylphospho-beta-D-ribofuranose + phosphate
-
 ?
3.1.3.111 trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv trans,octacis-decaprenylphospho-beta-D-ribofuranose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.111 ? x * 20000, His6-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis
3.1.3.111 ? x * 17200, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.45 5-phosphoribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase
-
 Mycobacterium tuberculosis
2.4.2.45 Rv3806c
-
 Mycobacterium tuberculosis
3.1.3.111 Rv3807c
-
 Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.111 Mycobacterium tuberculosis calculated from amino acid sequence
-
 12.3