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Literature summary extracted from

  • Wang, H.; Zhang, C.; Chen, H.; Yang, Q.; Zhou, X.; Gu, Z.; Zhang, H.; Chen, W.; Chen, Y.Q.
    Characterization of an fungal L-fucokinase involved in Mortierella alpina GDP-L-fucose salvage pathway (2016), Glycobiology, 26, 880-887.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.52 expressed in Escherichia coli BL21(DE3) cells Mortierella alpina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.52 Co2+ complete inhibition at 20 mM Mortierella alpina
2.7.1.52 EDTA
-
 Mortierella alpina
2.7.1.52 Fe2+ complete inhibition at 20 mM Mortierella alpina
2.7.1.52 GDP-L-fucose less than 30% inhibition at 0.2 mM Mortierella alpina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 1.59
-
 L-fucose at pH 7.4 and 37°C Mortierella alpina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Mg2+ best stimulation of activity in the presence of 2 mM Mg2+ Mortierella alpina
2.7.1.52 Mn2+ may replace Mg2+ Mortierella alpina
2.7.1.52 additional information not influenced by Ca2+, Cu2+, K+, Hg2+, Ni2+ and Zn2+ Mortierella alpina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 49000
-
 x * 49000, SDS-PAGE Mortierella alpina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + L-fucose Mortierella alpina
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose Mortierella alpina ATCC 32222
-
 ADP + beta-L-fucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Mortierella alpina
-
-
-
2.7.1.52 Mortierella alpina ATCC 32222
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52 nickel affinity column chromatography Mortierella alpina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + L-fucose
-
 Mortierella alpina ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose
-
 Mortierella alpina ATCC 32222 ADP + beta-L-fucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.52 ? x * 49000, SDS-PAGE Mortierella alpina

Synonyms

EC Number Synonyms Comment Organism
2.7.1.52 Fuk
-
 Mortierella alpina
2.7.1.52 L-fucokinase
-
 Mortierella alpina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.52 37
-
-
 Mortierella alpina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.52 25 50 more than 50% activity between 25 and 50°C Mortierella alpina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.52 4 37 the enzyme is stable for 2 h at 4 and 12°C. At 20°C, the enzyme shows 80% activity after 1 h and less than 20% activity after 2 h incubation. At 28°C, the enzyme is stable for 30 min, while at 37°C, the enzyme activity drops to about 37% after 30 min Mortierella alpina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.52 23.35
-
 L-fucose at pH 7.4 and 37°C Mortierella alpina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.52 7.4
-
-
 Mortierella alpina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.52 6 10 more than 50% activity between pH 6.0 and 10.0 Mortierella alpina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.52 3 12 After 30 min at pH 3,0, 5.0, 7.0, 8.0, 10.0, and 12.0, the enzyme shows about 25%, 50%, 90%, 100%, 75%, and 35% activity, respectively Mortierella alpina

Expression

EC Number Organism Comment Expression
2.7.1.52 Mortierella alpina the enzyme transcript level is up-regulated 4.3fold by nitrogen exhaustion when Mortierella alpina starts to accumulate lipid up