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Literature summary extracted from

  • Kajiura, H.; Hamaguchi, Y.; Mizushima, H.; Misaki, R.; Fujiyama, K.
    Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active alpha2,6-sialyltransferase (2015), Glycobiology, 25, 1441-1453.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 EDTA 138% activity at 10 mM Bombyx mori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expressed in Sf9 insect cells Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.1 N284A the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.3.1 N284Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 CTP complete inhibition at 10 mM Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.24
-
 CMP-N-acetylneuraminate wild type enzyme, at pH 6.0 and 37°C Bombyx mori
2.4.3.1 1.18
-
 CMP-N-acetylneuraminate mutant enzyme N284Q, at pH 6.0 and 37°C Bombyx mori
2.4.3.1 2.02
-
 CMP-N-acetylneuraminate mutant enzyme N284A, at pH 6.0 and 37°C Bombyx mori
2.4.3.1 35.7
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl mutant enzyme N284Q, at pH 6.0 and 37°C Bombyx mori
2.4.3.1 40.2
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl wild type enzyme, at pH 6.0 and 37°C Bombyx mori
2.4.3.1 52.8
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl mutant enzyme N284A, at pH 6.0 and 37°C Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 Golgi apparatus
-
 Bombyx mori 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.1 additional information the enzyme does not require ions for activity Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 42000
-
 x * 42000, SDS-PAGE Bombyx mori
2.4.3.1 53100
-
 x * 53100, calculated from amino acid sequence Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Bombyx mori
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 Co2+ affinity column chromatography, and gel filtration Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 fat body
-
 Bombyx mori
-
2.4.3.1 larva
-
 Bombyx mori
-
2.4.3.1 malpighian tubule
-
 Bombyx mori
-
2.4.3.1 midgut
-
 Bombyx mori
-
2.4.3.1 silk gland
-
 Bombyx mori
-
2.4.3.1 testis
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + (Galbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc 34.9 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + (GalNAcbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc 140 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide 2.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide 100% activity Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + 4-nitrophenyl N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide best acceptor with 371.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialofetuin
-
 Bombyx mori ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Bombyx mori CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + Galbeta-4-nitrophenyl 26.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + GalNAcbeta-4-nitrophenyl 120.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
2.4.3.1 additional information no activity towards Glcalpha-4-nitrophenyl, Galalpha-4-nitrophenyl, GalNAcalpha-4-nitrophenyl, Galbeta(1,3)-GalNAcbeta-4-nitrophenyl and GalNAcbeta(1,3)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 ? x * 42000, SDS-PAGE Bombyx mori
2.4.3.1 ? x * 53100, calculated from amino acid sequence Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha2,6-sialyltransferase
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 30
-
-
 Bombyx mori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.3.1 25 35
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.3.1
-
 30 the enzyme is stable below 30°C for 1 h but shows significantly decreased activities at higher temperatures Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 8
-
-
 Bombyx mori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.1 6 9
-
 Bombyx mori