EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | EDTA | 138% activity at 10 mM | Bombyx mori |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.1 | expressed in Sf9 insect cells | Bombyx mori |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.3.1 | N284A | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
2.4.3.1 | N284Q | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | CTP | complete inhibition at 10 mM | Bombyx mori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.24 | - |
CMP-N-acetylneuraminate | wild type enzyme, at pH 6.0 and 37°C | Bombyx mori | |
2.4.3.1 | 1.18 | - |
CMP-N-acetylneuraminate | mutant enzyme N284Q, at pH 6.0 and 37°C | Bombyx mori | |
2.4.3.1 | 2.02 | - |
CMP-N-acetylneuraminate | mutant enzyme N284A, at pH 6.0 and 37°C | Bombyx mori | |
2.4.3.1 | 35.7 | - |
Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | mutant enzyme N284Q, at pH 6.0 and 37°C | Bombyx mori | |
2.4.3.1 | 40.2 | - |
Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | wild type enzyme, at pH 6.0 and 37°C | Bombyx mori | |
2.4.3.1 | 52.8 | - |
Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | mutant enzyme N284A, at pH 6.0 and 37°C | Bombyx mori |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.3.1 | Golgi apparatus | - |
Bombyx mori | 5794 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | additional information | the enzyme does not require ions for activity | Bombyx mori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 42000 | - |
x * 42000, SDS-PAGE | Bombyx mori |
2.4.3.1 | 53100 | - |
x * 53100, calculated from amino acid sequence | Bombyx mori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine | Bombyx mori | - |
CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.1 | Bombyx mori | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.3.1 | Co2+ affinity column chromatography, and gel filtration | Bombyx mori |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.1 | fat body | - |
Bombyx mori | - |
2.4.3.1 | larva | - |
Bombyx mori | - |
2.4.3.1 | malpighian tubule | - |
Bombyx mori | - |
2.4.3.1 | midgut | - |
Bombyx mori | - |
2.4.3.1 | silk gland | - |
Bombyx mori | - |
2.4.3.1 | testis | - |
Bombyx mori | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | CMP-N-acetylneuraminate + (Galbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc | 34.9 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | ? | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + (GalNAcbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc | 140 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | ? | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide | 2.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide | 100% activity | Bombyx mori | CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + 4-nitrophenyl N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide | best acceptor with 371.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + asialofetuin | - |
Bombyx mori | ? | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine | - |
Bombyx mori | CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + Galbeta-4-nitrophenyl | 26.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | ? | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + GalNAcbeta-4-nitrophenyl | 120.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | ? | - |
? | |
2.4.3.1 | additional information | no activity towards Glcalpha-4-nitrophenyl, Galalpha-4-nitrophenyl, GalNAcalpha-4-nitrophenyl, Galbeta(1,3)-GalNAcbeta-4-nitrophenyl and GalNAcbeta(1,3)-GlcNAcbeta-4-nitrophenyl | Bombyx mori | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.3.1 | ? | x * 42000, SDS-PAGE | Bombyx mori |
2.4.3.1 | ? | x * 53100, calculated from amino acid sequence | Bombyx mori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.1 | alpha2,6-sialyltransferase | - |
Bombyx mori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 30 | - |
- |
Bombyx mori |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 25 | 35 | - |
Bombyx mori |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | - |
30 | the enzyme is stable below 30°C for 1 h but shows significantly decreased activities at higher temperatures | Bombyx mori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 8 | - |
- |
Bombyx mori |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 6 | 9 | - |
Bombyx mori |