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Literature summary extracted from

  • Choi, Y.H.; Kim, J.H.; Park, J.H.; Lee, N.; Kim, D.H.; Jang, K.S.; Park, I.H.; Kim, B.G.
    Protein engineering of alpha2,3/2,6-sialyltransferase to improve the yield and productivity of in vitro sialyllactose synthesis (2014), Glycobiology, 24, 159-169.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expressed in Escherichia coli BW25113 (DE3) cells Photobacterium damselae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.1 I411A the mutant exhibits 169% of wild type activity Photobacterium damselae
2.4.3.1 I411T/L433T the mutant exhibits 5fold enhancement of the enzyme's specific activity compared with the wild type enzyme Photobacterium damselae
2.4.3.1 K135A the mutant exhibits 8% of wild type activity Photobacterium damselae
2.4.3.1 L112A the mutant exhibits 61% of wild type activity Photobacterium damselae
2.4.3.1 L433A the mutant exhibits 114% of wild type activity Photobacterium damselae
2.4.3.1 L433S the mutant exhibits 3fold enhancement of the enzyme's specific activity compared with the wild type enzyme Photobacterium damselae
2.4.3.1 L433T the mutant exhibits 3fold enhancement of the enzyme's specific activity compared with the wild type enzyme Photobacterium damselae
2.4.3.1 N157A the mutant exhibits 32% of wild type activity Photobacterium damselae
2.4.3.1 R136A the mutant exhibits 10% of wild type activity Photobacterium damselae
2.4.3.1 S142A the mutant exhibits 38% of wild type activity Photobacterium damselae
2.4.3.1 T344A the mutant exhibits 38% of wild type activity Photobacterium damselae
2.4.3.1 W347A the mutant exhibits 16% of wild type activity Photobacterium damselae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 2.27
-
 CMP-N-acetylneuraminate mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 3.37
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 5.87
-
 CMP-N-acetylneuraminate wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 6.06
-
 CMP-N-acetylneuraminate mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 7.62
-
 lactose wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 8.15
-
 CMP-N-acetylneuraminate mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 10.7
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 27.3
-
 lactose mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 59.7
-
 lactose mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 61.1
-
 lactose mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 62
-
 lactose mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 75.7
-
 lactose mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Photobacterium damselae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 Ni-NTA column chromatography Photobacterium damselae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Photobacterium damselae CMP + 6'-sialyllactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha 2,6-sialyltransferase
-
 Photobacterium damselae
2.4.3.1 alpha2,6ST
-
 Photobacterium damselae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.1 4.3
-
 lactose wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 4.32
-
 CMP-N-acetylneuraminate wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 10.7
-
 CMP-N-acetylneuraminate mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 10.9
-
 CMP-N-acetylneuraminate mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 16.9
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 18.8
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 20.3
-
 CMP-N-acetylneuraminate mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 25.4
-
 lactose mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 76.5
-
 lactose mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 85
-
 lactose mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 102
-
 lactose mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 110
-
 lactose mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.1 0.58
-
 lactose wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 0.74
-
 CMP-N-acetylneuraminate wild type enzyme, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 0.93
-
 lactose mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.26
-
 lactose mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.35
-
 lactose mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.43
-
 lactose mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.58
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.78
-
 lactose mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 1.8
-
 CMP-N-acetylneuraminate mutant enzyme I411T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 2.61
-
 CMP-N-acetylneuraminate mutant enzyme L433S, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 4.7
-
 CMP-N-acetylneuraminate mutant enzyme L433T, at pH 8.0 and 22°C Photobacterium damselae
2.4.3.1 5.6
-
 CMP-N-acetylneuraminate mutant enzyme I411T/L433T, at pH 8.0 and 22°C Photobacterium damselae