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Literature summary extracted from

  • Sharma, V.; Colson, P.; Giorgi, R.; Pontarotti, P.; Raoult, D.
    DNA-dependent RNA polymerase detects hidden giant viruses in published databanks (2014), Genome Biol. Evol., 6, 1603-1610.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.6 molecular biology usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies Acanthamoeba polyphaga Mimivirus
2.7.7.6 molecular biology usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies Phaeocystis globosa virus
2.7.7.6 molecular biology usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies Phytophthora parasitica virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 RNAPsubunit 2, RNAP2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis and tree. Reconstruction of putative ancestral RNAP2 from the genome of eukaryote Hydra magnipapillata and Phytophthora parasitica using viral RNAP2, detection of megaviruse DNAs that are integrated into eukaryote genomes and misclassified Acanthamoeba polyphaga Mimivirus
2.7.7.6 RNAPsubunit 2, RNAP2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis and tree. Reconstruction of putative ancestral RNAP2 from the genome of eukaryote Hydra magnipapillata and Phytophthora parasitica using viral RNAP2, detection of megaviruse DNAs that are integrated into eukaryote genomes and misclassified Phaeocystis globosa virus
2.7.7.6 RNAPsubunit 2, RNAP2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis and tree. Reconstruction of putative ancestral RNAP2 from the genome of eukaryote Hydra magnipapillata and Phytophthora parasitica using viral RNAP2, detection of megaviruse DNAs that are integrated into eukaryote genomes and misclassified Phytophthora parasitica virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Acanthamoeba polyphaga Mimivirus Q5UQ32 AND E3VZD2 subunit 6 and subunit beta
-
2.7.7.6 Phaeocystis globosa virus R4THW7 AND R4TFI0 DNA-directed RNA polymerase II subunit RPB6 and subunit RPB10
-
2.7.7.6 Phytophthora parasitica virus
-
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNAP
-
 Acanthamoeba polyphaga Mimivirus
2.7.7.6 RNAP
-
 Phaeocystis globosa virus
2.7.7.6 RNAP
-
 Phytophthora parasitica virus
2.7.7.6 RNAP2
-
 Acanthamoeba polyphaga Mimivirus
2.7.7.6 RNAP2
-
 Phaeocystis globosa virus
2.7.7.6 RNAP2
-
 Phytophthora parasitica virus

General Information

EC Number General Information Comment Organism
2.7.7.6 additional information DNA-dependent RNA polymerase (RNAP) genes are universal in microbes and conserved in giant viruses and may replace rDNA for identifying microbes Acanthamoeba polyphaga Mimivirus
2.7.7.6 additional information DNA-dependent RNA polymerase (RNAP) genes are universal in microbes and conserved in giant viruses and may replace rDNA for identifying microbes Phaeocystis globosa virus
2.7.7.6 additional information DNA-dependent RNA polymerase (RNAP) genes are universal in microbes and conserved in giant viruses and may replace rDNA for identifying microbes Phytophthora parasitica virus