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Literature summary extracted from

  • Doamekpor, S.K.; Sanchez, A.M.; Schwer, B.; Shuman, S.; Lima, C.D.
    How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes (2014), Genes Dev., 28, 1323-1336.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.50 GTase in complex with C-terminal domain repeats of RNA polymerase Pol2 reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2 C-terminal domain segment, S5PPSYSPTS5P, bracketed by two Ser5-PO4 marks. At least one of the two Ser5-PO4-binding sites is required for cell viability and each site is important for cell growth at 37°C. GTase binds the Spt5 C-terminal domain at a separate docking site in the OB-fold domain that captures the Trp4 residue of the Spt5 nonapeptide repeat T1PAW4NSGSK Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.50 G164T 56ld increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe
2.7.7.50 G164T/H201N 349foldd increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe
2.7.7.50 H201N 46ld increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe
2.7.7.50 H201N/R364A/Y368F 98fold increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe
2.7.7.50 R157E 85fold increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe
2.7.7.50 R157E/H201N 526fold increase in Kd value for Pol2 CTD–Ser5-PO4 Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.50 Schizosaccharomyces pombe P40997 mRNA-capping enzyme subunit alpha
-
2.7.7.50 Schizosaccharomyces pombe ATCC 24843 P40997 mRNA-capping enzyme subunit alpha
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.50 Ceg1
-
 Schizosaccharomyces pombe
2.7.7.50 GTase
-
 Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
2.7.7.50 physiological function a disruptive mutation in the polypeptide Spt5 C-terminal domain-binding site of GTase is synthetically lethal with mutations in the polymerase Pol2 C-terminal domain-binding site. C-terminal domain phosphorylation has opposite effects on the interaction of GTase with Pol2 (Ser5-PO4 is required for binding) versus Spt5 (Thr1-PO4 inhibits binding) Schizosaccharomyces pombe