Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ravanal, M.C.; Eyzaguirre, J.
    Heterologous expression and characterization of alpha-L-arabinofuranosidase 4 from Penicillium purpurogenum and comparison with the other isoenzymes produced by the fungus (2015), Fungal Biol., 119, 641-647.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.55 degradation enzyme shows synergistic effect on arabinose liberation from wheat arabinoxylan when combined with endoxylanase from Penicillium purpurogenum Talaromyces purpureogenus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expression in Pichia pastoris Talaromyces purpureogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 1.58
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.6, 37°C Talaromyces purpureogenus
3.2.1.55 5.3
-
 4-nitrophenyl beta-D-galactofuranoside pH 4.6, 37°C Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 55400
-
-
 Talaromyces purpureogenus
3.2.1.55 68000
-
-
 Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Talaromyces purpureogenus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-galactofuranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + beta-D-galactofuranose
-
 ?
3.2.1.55 alpha-1,5-L-arabinobiose + H2O
-
 Talaromyces purpureogenus 2 L-arabinose
-
 ?
3.2.1.55 alpha-1,5-L-arabinohexaose + 5 H2O
-
 Talaromyces purpureogenus 6 L-arabinose
-
 ?
3.2.1.55 additional information no substrates: short arabinooligosaccharides or debranched arabinan Talaromyces purpureogenus ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O
-
 Talaromyces purpureogenus L-arabinofuranose + ? release of 24.5% of L-arabinofuranose ?
3.2.1.55 wheat flour arabinoxylan + H2O
-
 Talaromyces purpureogenus L-arabinofuranose + ? release of 23% of L-arabinofuranose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 55400, calculated for mature protein, x * 68000, SDS-PAGE Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Abf4
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50
-
-
 Talaromyces purpureogenus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 1
-
 4-nitrophenyl beta-D-galactofuranoside pH 4.6, 37°C Talaromyces purpureogenus
3.2.1.55 2.1
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.6, 37°C Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.6
-
-
 Talaromyces purpureogenus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 5
-
 sharp decrease in activity above Talaromyces purpureogenus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 0.19
-
 4-nitrophenyl beta-D-galactofuranoside pH 4.6, 37°C Talaromyces purpureogenus
3.2.1.55 1.32
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.6, 37°C Talaromyces purpureogenus