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Literature summary extracted from

  • Pickl, A.; Johnsen, U.; Archer, R.; Schönheit, P.
    Identification and characterization of 2-keto-3-deoxygluconate kinase and 2-keto-3-deoxygalactonate kinase in the haloarchaeon Haloferax volcanii (2014), FEMS Microbiol. Lett., 361, 76-83.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.3
-
 ATP at pH 8.0 and 42°C Haloferax volcanii
2.7.1.11 8.8
-
 2-dehydro-3-deoxy-D-gluconate at pH 8.0 and 42°C Haloferax volcanii
2.7.1.58 0.2
-
 2-dehydro-3-deoxy-D-galactonate at pH 8.0 and 42°C Haloferax volcanii
2.7.1.58 0.3
-
 ATP at pH 8.0 and 42°C Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 35000
-
 6 * 35000, SDS-PAGE Haloferax volcanii
2.7.1.11 38000
-
-
 Haloferax volcanii
2.7.1.11 194000
-
 gel filtration Haloferax volcanii
2.7.1.58 35000
-
 6 * 35000, SDS-PAGE Haloferax volcanii
2.7.1.58 38000
-
-
 Haloferax volcanii
2.7.1.58 194000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + 2-dehydro-3-deoxy-D-gluconate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Haloferax volcanii
-
-
-
2.7.1.11 Haloferax volcanii D4GR05
-
-
2.7.1.45 Haloferax volcanii D4GSE6
-
-
2.7.1.58 Haloferax volcanii D4GR05
-
-
2.7.1.178 Haloferax volcanii D4GR05
-
-
2.7.1.178 Haloferax volcanii D4GSE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 Ni-NTA column chromatography and gel filtration Haloferax volcanii
2.7.1.58 Ni-NTA column chromatography and gel filtration Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.11 additional information the enzyme does also use 2-dehydro-3-deoxy-D-galactonate as substrate Haloferax volcanii ?
-
 ?
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 additional information 2-dehydro-3-deoxy-D-gluconate is a weak substrate Haloferax volcanii ?
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 homohexamer 6 * 35000, SDS-PAGE Haloferax volcanii
2.7.1.11 homohexamer 6 * 38000, CTAB-PAGE Haloferax volcanii
2.7.1.58 homohexamer 6 * 35000, SDS-PAGE Haloferax volcanii
2.7.1.58 homohexamer 6 * 38000, CTAB-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 2-keto-3-deoxygluconate kinase
-
 Haloferax volcanii
2.7.1.11 HVO_A0328
-
 Haloferax volcanii
2.7.1.11 KDG kinase
-
 Haloferax volcanii
2.7.1.11 KDGal kinase
-
 Haloferax volcanii
2.7.1.11 KDGK-1
-
 Haloferax volcanii
2.7.1.11 KDGK-2
-
 Haloferax volcanii
2.7.1.45 2-keto-3-deoxygluconate kinase
-
 Haloferax volcanii
2.7.1.45 HVO_0549
-
 Haloferax volcanii
2.7.1.45 KDG kinase
-
 Haloferax volcanii
2.7.1.45 KDGK-1
-
 Haloferax volcanii
2.7.1.58 2-keto-3-deoxygluconate kinase
-
 Haloferax volcanii
2.7.1.58 KDGal kinase
-
 Haloferax volcanii
2.7.1.58 KDGK-2
-
 Haloferax volcanii
2.7.1.178 2-keto-3-deoxygalactonate kinase
-
 Haloferax volcanii
2.7.1.178 2-keto-3-deoxygluconate kinase
-
 Haloferax volcanii
2.7.1.178 HVO_0549 locus name Haloferax volcanii
2.7.1.178 HVO_A0328 locus name Haloferax volcanii
2.7.1.178 KDGK-1
-
 Haloferax volcanii
2.7.1.178 KDGK-2
-
 Haloferax volcanii

General Information

EC Number General Information Comment Organism
2.7.1.178 metabolism KDGK-1 represents the functional constitutively expressed 2-keto-3-deoxygluconate kinase in glucose degradation, whereas KDGK-2 is an inducible 2-keto-3-deoxygalactonate kinase likely involved in D-galactose catabolism Haloferax volcanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.11 8
-
 2-dehydro-3-deoxy-D-gluconate at pH 8.0 and 42°C Haloferax volcanii
2.7.1.58 432
-
 2-dehydro-3-deoxy-D-galactonate at pH 8.0 and 42°C Haloferax volcanii