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Literature summary extracted from

  • Van Ekert, E.; Shatters, R.G.; Rouge, P.; Powell, C.A.; Smagghe, G.; Borovsky, D.
    Cloning and expressing a highly functional and substrate specific farnesoic acid O-methyltransferase from the Asian citrus psyllid (Diaphorina citri Kuwayama) (2015), FEBS Open Bio, 5, 264-275.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.325 expressed in Escherichia coli One Shot BL21 (DE3) cells Diaphorina citri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.325 Zn2+ complete inhibition at 1 mM Diaphorina citri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.325 0.00002
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00046
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00253
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00458
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00565
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.325 additional information does not require Ca2+, Mg2+ or Zn2+ Diaphorina citri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.325 31000
-
 x * 31000, SDS-PAGE Diaphorina citri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate Diaphorina citri preferred substrate S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.325 Diaphorina citri A0A075W3Z0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.325 Ni-NTA column chromatography Diaphorina citri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.325 corpora allata
-
 Diaphorina citri
-
2.1.1.325 male accessory gland
-
 Diaphorina citri
-
2.1.1.325 ovary
-
 Diaphorina citri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate preferred substrate Diaphorina citri S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + (2E,6Z)-10cis-juvenile hormone I acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone I
-
 ?
2.1.1.325 S-adenosyl-L-methionine + (2Z,6E)-10cis-juvenile hormone I acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone I
-
 ?
2.1.1.325 S-adenosyl-L-methionine + homofarnesoate
-
 Diaphorina citri S-adenosyl-L-homocysteine + methylhomofarnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.325 ? x * 31000, SDS-PAGE Diaphorina citri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.325 FA-o-methyl transferase
-
 Diaphorina citri
2.1.1.325 farnesoic acid O-methyltransferase
-
 Diaphorina citri
2.1.1.325 fmtD
-
 Diaphorina citri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.325 0.000003
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.000008
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.000013
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00022
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00075
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.325 0.00066
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.00514
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 0.1434
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 1.645
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri
2.1.1.325 10.85
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri