Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pandey, T.; Chhetri, G.; Chinta, R.; Kumar, B.; Singh, D.B.; Tripathi, T.; Singh, A.K.
    Functional classification and biochemical characterization of a novel rho class glutathione S-transferase in Synechocystis PCC 6803 (2015), FEBS Open Bio, 5, 1-7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli M15 cells Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.14
-
 Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.
2.5.1.18 1.5
-
 glutathione at pH 7.5 and 30°C Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 30000
-
 x * 30000, SDS-PAGE Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni-NTA agarose column chromatography, and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Synechocystis sp. S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 dichloroacetate + glutathione
-
 Synechocystis sp. glyoxylate + HCl + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 30000, SDS-PAGE Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 rho class glutathione S-transferase
-
 Synechocystis sp.
2.5.1.18 sll1545
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
-
 Synechocystis sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 25 50 more than 60% activity between 25 and 50°C Synechocystis sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 15.48
-
 glutathione at pH 7.5 and 30°C Synechocystis sp.
2.5.1.18 82.17
-
 Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
-
 Synechocystis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 8.5 more than 40% activity between pH 6.0 and 8.5 Synechocystis sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 10.32
-
 glutathione at pH 7.5 and 30°C Synechocystis sp.
2.5.1.18 574.6
-
 Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.