Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tsuda, T.; Nihira, T.; Chiku, K.; Suzuki, E.; Arakawa, T.; Nishimoto, M.; Kitaoka, M.; Nakai, H.; Fushinobu, S.
    Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua (2015), FEBS Lett., 589, 3816-3821.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.339 expression in Escherichia coli as a C-terminally (His)6-tagged protein Listeria innocua

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.339 sitting drop vapor diffusion method at 20°C, crystals complexed with beta-1,2-Man2 and beta-1,2-Man3 are prepared by soaking in the cryoprotectant solution supplemented with 50 mM beta-1,2-Man2 or 10 mM beta-1,2-Man3. beta-1,2-Mannotriose is bound in a U-shape, interacting with a phosphate analog at both ends. Lin0857 has a unique dimer structure connected by a loop, and a significant open–close loop displacement iss observed for substrate entry. A long loop, which is exclusively present in Lin0857, covers the active site to limit the pocket size. A structural basis for substrate recognition and phosphorolysis is provided Listeria innocua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.339 0.13
-
 phosphate 30°C, pH 7, cosubstrate: beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose Listeria innocua
2.4.1.339 0.19
-
 phosphate 30°C, pH 7, cosubstrate: beta-D-mannopyranosyl-(1->2)-D-mannopyranose Listeria innocua
2.4.1.339 0.44
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua
2.4.1.339 1.5
-
 D-fructose 30°C, pH 6 Listeria innocua
2.4.1.339 2.1
-
 D-mannopyranose 30°C, pH 6 Listeria innocua
2.4.1.339 3.1
-
 alpha-D-mannose 1-phosphate 30°C, pH 6 Listeria innocua
2.4.1.339 4.1
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua
2.4.1.339 5.1
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 6 Listeria innocua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.339 40000
-
 2 * 40000, SDS-PAGE Listeria innocua
2.4.1.339 88000
-
 gel filtration Listeria innocua

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.339 Listeria innocua Q92DF6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.339
-
 Listeria innocua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.339 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate the enzyme preferentially phosphorolyzes beta-1,2-Man2 over beta-1,2-Man3 Listeria innocua beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.339 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannose 1-phosphate no production of tetrasaccharide is detected Listeria innocua beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
-
 r
2.4.1.339 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate the enzyme preferentially phosphorolyzes beta-1,2-Man2 over beta-1,2-Man3 Listeria innocua D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.339 D-fructose + alpha-D-mannose 1-phosphate
-
 Listeria innocua ? + phosphate
-
 r
2.4.1.339 D-mannopyranose + alpha-D-mannose 1-phosphate
-
 Listeria innocua beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.339 dimer 2 * 40000, SDS-PAGE Listeria innocua

Synonyms

EC Number Synonyms Comment Organism
2.4.1.339 Lin0857
-
 Listeria innocua

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.339 50
-
 stable up to 50°C during 15 min of incubation Listeria innocua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.339 3.9
-
 D-fructose 30°C, pH 6 Listeria innocua
2.4.1.339 12
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 6 Listeria innocua
2.4.1.339 12
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua
2.4.1.339 31
-
 D-mannopyranose 30°C, pH 6 Listeria innocua
2.4.1.339 33
-
 alpha-D-mannose 1-phosphate 30°C, pH 6 Listeria innocua
2.4.1.339 35
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.339 6
-
 synthetic reaction Listeria innocua
2.4.1.339 7
-
 phosphorolytic reaction Listeria innocua

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.339 4.5 7.5 pH 4.5: about 60% of maximal activity, pH 7.5: about 60% of maximal activity, synthetic reaction Listeria innocua
2.4.1.339 5.5 8 pH 5.5: about 75% of maximal activity, pH 8.0: about 60% of maximal activity, phosphorolytic reaction Listeria innocua

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.339 5.5 9 4°C, 24 h, stable Listeria innocua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.339 2.3
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 6 Listeria innocua
2.4.1.339 2.7
-
 D-fructose 30°C, pH 6 Listeria innocua
2.4.1.339 3
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua
2.4.1.339 11
-
 alpha-D-mannose 1-phosphate 30°C, pH 6 Listeria innocua
2.4.1.339 15
-
 D-mannopyranose 30°C, pH 6 Listeria innocua
2.4.1.339 79
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose 30°C, pH 7 Listeria innocua