EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.6 | D183A | inactive | Lactococcus lactis |
2.7.1.6 | D183N | inactive | Lactococcus lactis |
2.7.1.6 | R36A | 100fold decrease in activity | Lactococcus lactis |
2.7.1.6 | R36N | 100fold decrease in activity | Lactococcus lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.018 | - |
ATP | mutant R36K, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 0.053 | - |
ATP | wild-type, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 0.14 | - |
ATP | mutant R36A, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 0.48 | - |
alpha-D-galactose | wild-type, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 1 | - |
alpha-D-galactose | mutant R36K, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 1.8 | - |
alpha-D-galactose | mutant R36A, pH 8.0, 24°C | Lactococcus lactis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.6 | Lactococcus lactis | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.6 | ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate | mechanism of the reaction is sequential in both directions. Residue Asp183 acts as a catalytic base that deprotonates the C1 hydroxyl group of galactose. The pH-kcat profile of the synthesis of galactose 1-phosphate has a pKa of 6.9 likely due to Asp183. The pH-kcat/KGal profile of the reverse reaction lacks a key pKa required for a direct proton transfer mechanism. Arg36 may lower the pKa of the C1 hydroxyl to facilitate deprotonation | Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.6 | 0.0043 | - |
mutant R36K, synthesis of ATP, pH 8.0, 24°C | Lactococcus lactis |
2.7.1.6 | 0.0067 | - |
mutant R36A, synthesis of ATP, pH 8.0, 24°C | Lactococcus lactis |
2.7.1.6 | 0.08 | - |
mutant R36A, synthesis of galactose 1-phosphate, pH 8.0, 24°C | Lactococcus lactis |
2.7.1.6 | 0.1 | - |
mutant R36K, synthesis of galactose 1-phosphate, pH 8.0, 24°C | Lactococcus lactis |
2.7.1.6 | 0.43 | - |
wild-type, synthesis of ATP, pH 8.0, 24°C | Lactococcus lactis |
2.7.1.6 | 11.5 | - |
wild-type, synthesis of galactose 1-phosphate, pH 8.0, 24°C | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.6 | ADP + alpha-D-galactose 1-phosphate | - |
Lactococcus lactis | ATP + alpha-D-galactose | - |
r | |
2.7.1.6 | ATP + alpha-D-galactose | - |
Lactococcus lactis | ADP + alpha-D-galactose 1-phosphate | - |
r | |
2.7.1.6 | additional information | mechanism of the reaction is sequential in both directions. Residue Asp183 acts as a catalytic base that deprotonates the C1 hydroxyl group of galactose. The pH-kcat profile of the synthesis of galactose 1-phosphate has a pKa of 6.9 likely due to Asp183. The pH-kcat/KGal profile of the reverse reaction lacks a key pKa required for a direct proton transfer mechanism. Arg36 may lower the pKa of the C1 hydroxyl to facilitate deprotonation | Lactococcus lactis | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.15 | - |
alpha-D-galactose | mutant R36A, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 0.45 | - |
alpha-D-galactose | mutant R36K, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 11 | - |
alpha-D-galactose | wild-type, pH 8.0, 24°C | Lactococcus lactis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.081 | - |
alpha-D-galactose | mutant R36A, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 0.45 | - |
alpha-D-galactose | mutant R36K, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 1 | - |
ATP | mutant R36A, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 23 | - |
alpha-D-galactose | wild-type, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 25 | - |
ATP | mutant R36K, pH 8.0, 24°C | Lactococcus lactis | |
2.7.1.6 | 207 | - |
ATP | wild-type, pH 8.0, 24°C | Lactococcus lactis |