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Literature summary extracted from

  • Koenig, S.; Moreau, C.; Dupont, G.; Scoumanne, A.; Erneux, C.
    Regulation of NGF-driven neurite outgrowth by Ins(1,4,5)P3 kinase is specifically associated with the two isoenzymes Itpka and Itpkb in a model of PC12 cells (2015), FEBS J., 282, 2553-2569.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.127 additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKA, generation of Itpka N-terminal F-actin binding domain (N66-GFP) and the N66(L34P)-GFP mutants. N-terminal deletion mutants are fully active Rattus norvegicus
2.7.1.127 additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKB. N-terminal deletion mutants are fully active Rattus norvegicus
2.7.1.127 additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKC Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus P17105 gene Itpka
-
2.7.1.127 Rattus norvegicus P42335 gene Itpkb
-
2.7.1.127 Rattus norvegicus Q80ZG2 gene Itpkc
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 PC-12 cell
-
 Rattus norvegicus
-
2.7.1.127 pheochromocytoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.127 Ins(1,4,5)P3 kinase
-
 Rattus norvegicus
2.7.1.127 ITPKA
-
 Rattus norvegicus
2.7.1.127 Itpkb
-
 Rattus norvegicus
2.7.1.127 Itpkc
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.127 malfunction neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC-12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells Rattus norvegicus
2.7.1.127 malfunction neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells Rattus norvegicus
2.7.1.127 additional information an F-actin binding site is located in the N-terminal part of isozyme Itpka. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active Rattus norvegicus
2.7.1.127 additional information an F-actin binding site is located in the N-terminal part of isozyme Itpkb. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active Rattus norvegicus
2.7.1.127 additional information neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases Rattus norvegicus
2.7.1.127 physiological function isozyme Itpka inhibits neurite outgrowth through both F-actin binding Rattus norvegicus
2.7.1.127 physiological function isozyme Itpkb inhibits neurite outgrowth through both F-actin binding Rattus norvegicus
2.7.1.127 physiological function isozyme Itpkc does not influence neurite length and neuron growth or F-actin binding Rattus norvegicus