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Literature summary extracted from

  • Wang, Y.; Deng, Z.; Qu, X.
    Characterization of a SAM-dependent fluorinase from a latent biosynthetic pathway for fluoroacetate and 4-fluorothreonine formation in Nocardia brasiliensis (2014), F1000Res., 3, 61-73.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.63 EDTA
-
 Nocardia brasiliensis
2.5.1.63 EDTA
-
 Streptomyces cattleya

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.63 gene flA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type His6-tagged enzymes in Escherichia coli strain BL21(DE3) Streptomyces cattleya
2.5.1.63 gene nobA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His6-tagged enzymes in Escherichia coli strain BL21(DE3) Nocardia brasiliensis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 S158A site-directed mutagenesis, recombinant mutant protein NobA-S158A completely loses fluorination activity Nocardia brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.63 Cu2+
-
 Nocardia brasiliensis
2.5.1.63 Fe2+
-
 Nocardia brasiliensis
2.5.1.63 Zn2+
-
 Nocardia brasiliensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.21
-
 S-adenosyl-L-methionine pH 7.5, 26°C, recombinant enzyme Streptomyces cattleya
2.5.1.63 0.416
-
 S-adenosyl-L-methionine pH 7.5, 26°C, recombinant enzyme Nocardia brasiliensis
2.5.1.63 2.167
-
 fluoride pH 7.5, 26°C, recombinant enzyme Streptomyces cattleya
2.5.1.63 4.153
-
 fluoride pH 7.5, 26°C, recombinant enzyme Nocardia brasiliensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.63 Mg2+ slightly activating Nocardia brasiliensis
2.5.1.63 Mn2+ slightly activating Nocardia brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.63 34600
-
-
 Streptomyces cattleya
2.5.1.63 34700
-
-
 Nocardia brasiliensis
2.5.1.63 200000
-
 recombinant enzyme, gel filtration Nocardia brasiliensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Nocardia brasiliensis
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride Nocardia brasiliensis ATCC 700358
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya DSM 46488
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Nocardia brasiliensis W8JNL4 gene nobA
-
2.5.1.63 Nocardia brasiliensis ATCC 700358 W8JNL4 gene nobA
-
2.5.1.63 Streptomyces cattleya Q70GK9 gene flA
-
2.5.1.63 Streptomyces cattleya DSM 46488 Q70GK9 gene flA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.63 recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nocardia brasiliensis
2.5.1.63 recombinant wild-type His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Nocardia brasiliensis 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Nocardia brasiliensis ATCC 700358 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya DSM 46488 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.63 ? x * 34600 recombinant enzyme, SDS-PAGE Streptomyces cattleya
2.5.1.63 hexamer 6 * 34700 recombinant enzyme, SDS-PAGE Nocardia brasiliensis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 flA
-
 Streptomyces cattleya
2.5.1.63 fluorinase
-
 Nocardia brasiliensis
2.5.1.63 fluorinase
-
 Streptomyces cattleya
2.5.1.63 NobA
-
 Nocardia brasiliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 37
-
 the optimal temperature for fluorination reactivity is 37°C. At this temperature S-adenosyl-L-methionine degradation is also expedited. 26°C can provide acceptable activity and slow down the degradation of S-adenosyl-L-methionine and is therefore used for kinetic studies Nocardia brasiliensis
2.5.1.63 37
-
 the optimal temperature for fluorination reactivity is 37°C. At this temperature S-adenosyl-L-methionine degradation is also expedited. 26°C can provide acceptable activity and slow down the degradation of S-adenosyl-L-methionine and is therefore used for kinetic studies Streptomyces cattleya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.0012
-
 fluoride pH 7.5, 26°C, recombinant enzyme Nocardia brasiliensis
2.5.1.63 0.0018
-
 fluoride pH 7.5, 26°C, recombinant enzyme Streptomyces cattleya
2.5.1.63 0.0021
-
 S-adenosyl-L-methionine pH 7.5, 26°C, recombinant enzyme Streptomyces cattleya
2.5.1.63 0.0023
-
 S-adenosyl-L-methionine pH 7.5, 26°C, recombinant enzyme Nocardia brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.63 6.5
-
-
 Nocardia brasiliensis
2.5.1.63 7
-
-
 Streptomyces cattleya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.63 5 9.5 activity range, profile overview Nocardia brasiliensis
2.5.1.63 5.5 10 activity range, profile overview Streptomyces cattleya

General Information

EC Number General Information Comment Organism
2.5.1.63 evolution the enzyme belongs to the fluorinases group of the DUF62 enzyme family Nocardia brasiliensis
2.5.1.63 evolution the enzyme belongs to the fluorinases group of the DUF62 enzyme family Streptomyces cattleya
2.5.1.63 metabolism enzyme NobA shows similar halide specificity and characteristics to the fluorination enzyme FlA of the fl pathway Nocardia brasiliensis
2.5.1.63 metabolism enzyme NobA shows similar halide specificity and characteristics to the fluorination enzyme FlA of the fl pathway Streptomyces cattleya
2.5.1.63 additional information essential residue S158 of FlA plays critical role in fluoride binding and desolvation Streptomyces cattleya
2.5.1.63 additional information essential residue S158 of NbA is required for activity Nocardia brasiliensis