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Literature summary extracted from

  • Hamajima, Y.; Nagae, T.; Watanabe, N.; Ohmae, E.; Kato-Yamada, Y.; Kato, C.
    Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution (2016), Extremophiles, 20, 177-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expressed in Escherichia coli HB101 cells Shewanella benthica
1.1.1.85 expressed in Escherichia coli HB101 cells Shewanella oneidensis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 A266S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
1.1.1.85 A268I the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella oneidensis
1.1.1.85 I268A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
1.1.1.85 L106M the mutant shows increased catalytic efficiency compared to the wild type enzyme Shewanella oneidensis
1.1.1.85 M106L the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
1.1.1.85 S266A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.0122
-
 (2R,3S)-3-isopropylmalate mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.0122
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.0141
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.0226
-
 (2R,3S)-3-isopropylmalate mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.0239
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.0344
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.0345
-
 (2R,3S)-3-isopropylmalate mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.0356
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.0708
-
 NAD+ mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.0756
-
 NAD+ mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.079
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.0814
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 0.1058
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.1707
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.2379
-
 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 0.2785
-
 NAD+ mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Shewanella benthica
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Shewanella oneidensis
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Shewanella benthica DB21MT-2
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Shewanella oneidensis MR-1 / ATCC 700550
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Shewanella benthica
-
-
-
1.1.1.85 Shewanella benthica DB21MT-2
-
-
-
1.1.1.85 Shewanella oneidensis Q8E9N3
-
-
1.1.1.85 Shewanella oneidensis MR-1 / ATCC 700550 Q8E9N3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 Ni Sepharose 6 column chromatography Shewanella benthica
1.1.1.85 Ni Sepharose 6 column chromatography Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella benthica (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella oneidensis (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella benthica DB21MT-2 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella oneidensis MR-1 / ATCC 700550 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Shewanella benthica
1.1.1.85 IPMDH
-
 Shewanella oneidensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 2 3.7 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 9.6
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 10.3
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 14.8
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 15.2
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 15.6
-
 NAD+ mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 16.1
-
 (2R,3S)-3-isopropylmalate mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 16.4
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 16.7
-
 (2R,3S)-3-isopropylmalate mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 17.1
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 17.4
-
 NAD+ mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 17.4
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 18.7
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 20.4
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 48.1
-
 (2R,3S)-3-isopropylmalate mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
1.1.1.85 50.5
-
 NAD+ mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Shewanella benthica
1.1.1.85 NAD+
-
 Shewanella oneidensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.85 60
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 100
-
 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 180
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 190
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 270
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 590
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 620
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1.1.1.85 1350
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica