Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bibi, Z.; Qader, S.A.; Aman, A.
    Calcium alginate matrix increases the stability and recycling capability of immobilized endo-beta-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29 (2015), Extremophiles, 19, 819-827.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 degradation immobilization of enzyme within calcium alginate beads using entrapment technique. Temperature (50°C) and pH (7.0) optima of immobilized enzyme remain same, but enzyme-substrate reaction time increases from 5.0 to 30.0 min as compared to free enzyme. The diffusion limit of high molecular weight xylan (corncob) causes a decline in Vmax of immobilized enzyme from 4773 to 203.7 U/min, whereas the Km value increases from 0.5074 to 0.5722 mg/ml. Immobilized endo-beta-1,4-xylanase is stable even at high temperatures and retains 18 and 9% residual activity at 70°C and 80°C, respectively. The immobilized enzyme also exhibits sufficient recycling efficiency up to five reaction cycles Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 corncob xylan Km value of free enzyme is 0.507 mg/ml, of immobilized enzyme 0.572 mg/ml, pH 7.0, 50°C Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Geobacillus stearothermophilus
-
-
-
3.2.1.8 Geobacillus stearothermophilus KIBGE-IB29
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 corncob xylan + H2O
-
 Geobacillus stearothermophilus xylo-oligosaccharides + ?
-
 ?
3.2.1.8 corncob xylan + H2O
-
 Geobacillus stearothermophilus KIBGE-IB29 xylo-oligosaccharides + ?
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 70
-
 18% of maximum activity Geobacillus stearothermophilus
3.2.1.8 80
-
 9% of maximum activity Geobacillus stearothermophilus