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Literature summary extracted from

  • Uchida, Y.; Hayashi, H.; Washio, T.; Yamasaki, R.; Kato, S.; Oikawa, T.
    Cloning and characterization of a novel fold-type I branched-chain amino acid aminotransferase from the hyperthermophilic archaeon Thermococcus sp. CKU-1 (2014), Extremophiles, 18, 589-602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 expression in Escherichia coli Thermococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 Aminooxyacetate residual activity 79% Thermococcus sp.
2.6.1.42 DL-Penicillamine residual activity 79% Thermococcus sp.
2.6.1.42 hydroxylamine residual activity 72% Thermococcus sp.
2.6.1.42 Semicarbazide residual activity 95% Thermococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.034
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.06
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.06
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.15
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.16
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.18
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.34
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.55
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.73
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 0.83
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1.2
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1.7
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1.9
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 2
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 2.4
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 3.9
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 4.2
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 12
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Thermococcus sp. A0A060PQX5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 2-oxo-3-methylvalerate + glyoxalate
-
Thermococcus sp. L-isoleucine + glycine
-
r
2.6.1.42 DL-2-aminoheptanoate + 2-oxoglutarate
-
Thermococcus sp. 2-oxoheptanoate + L-glutamate
-
r
2.6.1.42 DL-2-aminooctanoate + 2-oxobutanoate
-
Thermococcus sp. 2-oxooctanoate + L-2-aminobutanoate
-
r
2.6.1.42 L-2-aminobutanoate + phenylpyruvate
-
Thermococcus sp. 2-oxobutanoate + L-phenylalanine
-
r
2.6.1.42 L-glutamate + glyoxalate
-
Thermococcus sp. 2-oxoglutarate + glycine
-
r
2.6.1.42 L-isoleucine + 2-oxoisocaproate
-
Thermococcus sp. 3-methyl-2-oxopentanoate + L-leucine
-
r
2.6.1.42 L-leucine + 2-oxobutanoate
-
Thermococcus sp. 2-oxoisohexanoate + L-valine
-
r
2.6.1.42 L-norleucine + 2-oxoisocaproate
-
Thermococcus sp. 2-oxohexanoate + L-leucine
-
r
2.6.1.42 L-norvaline + 2-oxocaproate
-
Thermococcus sp. 2-oxopentanoate + 2-aminohexanoate
-
r
2.6.1.42 L-phenylalanine + 2-oxovalerate
-
Thermococcus sp. phenylpyruvate + L-norvaline
-
r
2.6.1.42 L-valine + 2-oxoisovalerate
-
Thermococcus sp. 2-oxoisovalerate + L-leucine
-
r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
Thermococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.42 95
-
-
Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.42 90
-
stable for at least 5 h Thermococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.42 25
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 27
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 31
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 51
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 52
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 80
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 81
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 86
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 91
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 110
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 150
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 190
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 250
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 260
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 270
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 270
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 360
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 700
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 9.5
-
-
Thermococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.42 6 7
-
Thermococcus sp.

General Information

EC Number General Information Comment Organism
2.6.1.42 physiological function Thermococcus sp. CKU-1 requires L-leucine, L-methionine, L-phenylalanine, L-threonine, L-tryptophan, and L-tyrosine for its growth, which are good substrates for BcAT. The enzyme might be involved in the degradation of these amino acids Thermococcus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.42 11
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 13
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 19
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 21
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 38
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 39
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 45
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 87
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 110
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 150
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 350
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 430
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 460
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1200
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1500
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 1500
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 4600
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.6.1.42 11000
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.