EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.42 | expression in Escherichia coli | Thermococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | Aminooxyacetate | residual activity 79% | Thermococcus sp. | |
2.6.1.42 | DL-Penicillamine | residual activity 79% | Thermococcus sp. | |
2.6.1.42 | hydroxylamine | residual activity 72% | Thermococcus sp. | |
2.6.1.42 | Semicarbazide | residual activity 95% | Thermococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 0.034 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.06 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.06 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.15 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.16 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.18 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.34 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.55 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.73 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 0.83 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1.2 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1.7 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1.9 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 2 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 2.4 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 3.9 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 4.2 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 12 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.42 | Thermococcus sp. | A0A060PQX5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | 2-oxo-3-methylvalerate + glyoxalate | - |
Thermococcus sp. | L-isoleucine + glycine | - |
r | |
2.6.1.42 | DL-2-aminoheptanoate + 2-oxoglutarate | - |
Thermococcus sp. | 2-oxoheptanoate + L-glutamate | - |
r | |
2.6.1.42 | DL-2-aminooctanoate + 2-oxobutanoate | - |
Thermococcus sp. | 2-oxooctanoate + L-2-aminobutanoate | - |
r | |
2.6.1.42 | L-2-aminobutanoate + phenylpyruvate | - |
Thermococcus sp. | 2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.42 | L-glutamate + glyoxalate | - |
Thermococcus sp. | 2-oxoglutarate + glycine | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoisocaproate | - |
Thermococcus sp. | 3-methyl-2-oxopentanoate + L-leucine | - |
r | |
2.6.1.42 | L-leucine + 2-oxobutanoate | - |
Thermococcus sp. | 2-oxoisohexanoate + L-valine | - |
r | |
2.6.1.42 | L-norleucine + 2-oxoisocaproate | - |
Thermococcus sp. | 2-oxohexanoate + L-leucine | - |
r | |
2.6.1.42 | L-norvaline + 2-oxocaproate | - |
Thermococcus sp. | 2-oxopentanoate + 2-aminohexanoate | - |
r | |
2.6.1.42 | L-phenylalanine + 2-oxovalerate | - |
Thermococcus sp. | phenylpyruvate + L-norvaline | - |
r | |
2.6.1.42 | L-valine + 2-oxoisovalerate | - |
Thermococcus sp. | 2-oxoisovalerate + L-leucine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.42 | BcaT | - |
Thermococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 95 | - |
- |
Thermococcus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 90 | - |
stable for at least 5 h | Thermococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 25 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 27 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 31 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 51 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 52 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 80 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 81 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 86 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 91 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 110 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 150 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 190 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 250 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 260 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 270 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 270 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 360 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 700 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 9.5 | - |
- |
Thermococcus sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 6 | 7 | - |
Thermococcus sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.42 | physiological function | Thermococcus sp. CKU-1 requires L-leucine, L-methionine, L-phenylalanine, L-threonine, L-tryptophan, and L-tyrosine for its growth, which are good substrates for BcAT. The enzyme might be involved in the degradation of these amino acids | Thermococcus sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 11 | - |
L-2-aminobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 13 | - |
DL-2-aminoheptanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 19 | - |
L-phenylalanine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 21 | - |
L-norleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 38 | - |
L-valine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 39 | - |
L-glutamate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 45 | - |
L-norvaline | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 87 | - |
L-leucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 110 | - |
DL-2-aminooctanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 150 | - |
phenylpyruvate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 350 | - |
L-isoleucine | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 430 | - |
2-oxoglutarate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 460 | - |
2-oxobutanoate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1200 | - |
2-oxovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1500 | - |
2-oxoisovalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 1500 | - |
2-oxocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 4600 | - |
2-oxoisocaproate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. | |
2.6.1.42 | 11000 | - |
2-oxo-3-methylvalerate | half transamination reaction, pH 8.0, 90°C | Thermococcus sp. |