BRENDA - Enzyme Database

Molecular and biochemical characterisation of arginine kinases in Haemonchus contortus and Teladorsagia circumcincta

Umair, S.; Knight, J.S.; Bland, R.J.; Simpson, H.V.; Exp. Parasitol. 134, 362-367 (2013)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.7.3.3
medicine
the enzyme HcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm
Haemonchus contortus
2.7.3.3
medicine
the enzyme TcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm
Teladorsagia circumcincta
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.3.3
gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Haemonchus contortus
2.7.3.3
gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Teladorsagia circumcincta
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
agmatine
65% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
agmatine
61% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
canavanine
81% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
canavanine
79% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
citrulline
17% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
citrulline
21% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
Creatine
11% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
Creatine
9% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
glutamate
15% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
glutamate
21% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
glycine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
glycine
15% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
guanidine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
guanidine
12% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
isoleucine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
isoleucine
9% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
lysine
31% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
lysine
3% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
nitroarginine
30% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
nitroarginine
30% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
ornithine
27% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
ornithine
31% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
putrescine
15% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
putrescine
10% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
Urea
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
Urea
13% enzyme inhibition at 2 mM
Teladorsagia circumcincta
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.3
-
L-arginine
pH 7.5, 30°C, recombinant His-tagged enzyme
Teladorsagia circumcincta
2.7.3.3
0.35
-
L-arginine
pH 7.5, 30°C, recombinant His-tagged enzyme
Haemonchus contortus
2.7.3.3
0.8
-
ATP
pH 7.5, 30°C, recombinant His-tagged enzyme
Teladorsagia circumcincta
2.7.3.3
0.9
-
ATP
pH 7.5, 30°C, recombinant His-tagged enzyme
Haemonchus contortus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Cu2+
2 mM, results in 3% activity compared to Mg2+
Haemonchus contortus
2.7.3.3
Cu2+
2 mM, results in 11% activity compared to Mg2+
Teladorsagia circumcincta
2.7.3.3
Mg2+
2 mM, absolutely required, only 2% of maximal activity without Mg2+
Haemonchus contortus
2.7.3.3
Mg2+
2 mM, absolutely required, no activity without Mg2+
Teladorsagia circumcincta
2.7.3.3
Mn2+
2 mM, results in 41% activity compared to Mg2+
Haemonchus contortus
2.7.3.3
Mn2+
2 mM, results in 39% activity compared to Mg2+
Teladorsagia circumcincta
2.7.3.3
additional information
Zn2+ cannot substitute for Mg2+
Haemonchus contortus
2.7.3.3
additional information
Zn2+ cannot substitute for Mg2+
Teladorsagia circumcincta
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
46000
-
-
Haemonchus contortus
2.7.3.3
46000
-
-
Teladorsagia circumcincta
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-arginine
Teladorsagia circumcincta
-
ADP + Nomega-phospho-L-arginine
-
-
r
2.7.3.3
ATP + L-arginine
Haemonchus contortus
-
ADP + Nomega-phospho-L-arginine
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Haemonchus contortus
K0DG62
gene HcAK
-
2.7.3.3
Teladorsagia circumcincta
K0DDQ9
gene TcAK
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-arginine
-
738179
Teladorsagia circumcincta
ADP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
ATP + L-arginine
-
738179
Haemonchus contortus
ADP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
GTP + L-arginine
GTP shows 11% of the activity with ATP
738179
Haemonchus contortus
GDP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
GTP + L-arginine
GTP shows 7% of the activity with ATP
738179
Teladorsagia circumcincta
GDP + Nomega-phospho-L-arginine
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE
Haemonchus contortus
2.7.3.3
?
x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE
Teladorsagia circumcincta
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
30
-
assay at
Haemonchus contortus
2.7.3.3
30
-
assay at
Teladorsagia circumcincta
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
7.5
-
-
Haemonchus contortus
2.7.3.3
7.5
-
-
Teladorsagia circumcincta
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ADP
-
Haemonchus contortus
2.7.3.3
ADP
-
Teladorsagia circumcincta
2.7.3.3
ATP
-
Haemonchus contortus
2.7.3.3
ATP
-
Teladorsagia circumcincta
2.7.3.3
GTP
low activity
Haemonchus contortus
2.7.3.3
GTP
low activity
Teladorsagia circumcincta
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.3.3
medicine
the enzyme HcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm
Haemonchus contortus
2.7.3.3
medicine
the enzyme TcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm
Teladorsagia circumcincta
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Haemonchus contortus
2.7.3.3
gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Teladorsagia circumcincta
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ADP
-
Haemonchus contortus
2.7.3.3
ADP
-
Teladorsagia circumcincta
2.7.3.3
ATP
-
Haemonchus contortus
2.7.3.3
ATP
-
Teladorsagia circumcincta
2.7.3.3
GTP
low activity
Haemonchus contortus
2.7.3.3
GTP
low activity
Teladorsagia circumcincta
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
agmatine
65% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
agmatine
61% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
canavanine
81% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
canavanine
79% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
citrulline
17% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
citrulline
21% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
Creatine
11% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
Creatine
9% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
glutamate
15% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
glutamate
21% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
glycine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
glycine
15% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
guanidine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
guanidine
12% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
isoleucine
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
isoleucine
9% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
lysine
31% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
lysine
3% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
nitroarginine
30% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
nitroarginine
30% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
ornithine
27% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
ornithine
31% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
putrescine
15% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
putrescine
10% enzyme inhibition at 2 mM
Teladorsagia circumcincta
2.7.3.3
Urea
10% inhibition at 2 mM
Haemonchus contortus
2.7.3.3
Urea
13% enzyme inhibition at 2 mM
Teladorsagia circumcincta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.3
-
L-arginine
pH 7.5, 30°C, recombinant His-tagged enzyme
Teladorsagia circumcincta
2.7.3.3
0.35
-
L-arginine
pH 7.5, 30°C, recombinant His-tagged enzyme
Haemonchus contortus
2.7.3.3
0.8
-
ATP
pH 7.5, 30°C, recombinant His-tagged enzyme
Teladorsagia circumcincta
2.7.3.3
0.9
-
ATP
pH 7.5, 30°C, recombinant His-tagged enzyme
Haemonchus contortus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Cu2+
2 mM, results in 3% activity compared to Mg2+
Haemonchus contortus
2.7.3.3
Cu2+
2 mM, results in 11% activity compared to Mg2+
Teladorsagia circumcincta
2.7.3.3
Mg2+
2 mM, absolutely required, only 2% of maximal activity without Mg2+
Haemonchus contortus
2.7.3.3
Mg2+
2 mM, absolutely required, no activity without Mg2+
Teladorsagia circumcincta
2.7.3.3
Mn2+
2 mM, results in 41% activity compared to Mg2+
Haemonchus contortus
2.7.3.3
Mn2+
2 mM, results in 39% activity compared to Mg2+
Teladorsagia circumcincta
2.7.3.3
additional information
Zn2+ cannot substitute for Mg2+
Haemonchus contortus
2.7.3.3
additional information
Zn2+ cannot substitute for Mg2+
Teladorsagia circumcincta
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
46000
-
-
Haemonchus contortus
2.7.3.3
46000
-
-
Teladorsagia circumcincta
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-arginine
Teladorsagia circumcincta
-
ADP + Nomega-phospho-L-arginine
-
-
r
2.7.3.3
ATP + L-arginine
Haemonchus contortus
-
ADP + Nomega-phospho-L-arginine
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-arginine
-
738179
Teladorsagia circumcincta
ADP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
ATP + L-arginine
-
738179
Haemonchus contortus
ADP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
GTP + L-arginine
GTP shows 11% of the activity with ATP
738179
Haemonchus contortus
GDP + Nomega-phospho-L-arginine
-
-
-
r
2.7.3.3
GTP + L-arginine
GTP shows 7% of the activity with ATP
738179
Teladorsagia circumcincta
GDP + Nomega-phospho-L-arginine
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE
Haemonchus contortus
2.7.3.3
?
x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE
Teladorsagia circumcincta
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
30
-
assay at
Haemonchus contortus
2.7.3.3
30
-
assay at
Teladorsagia circumcincta
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
7.5
-
-
Haemonchus contortus
2.7.3.3
7.5
-
-
Teladorsagia circumcincta
General Information
EC Number
General Information
Commentary
Organism
2.7.3.3
evolution
phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi
Haemonchus contortus
2.7.3.3
evolution
phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi
Teladorsagia circumcincta
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.3.3
evolution
phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi
Haemonchus contortus
2.7.3.3
evolution
phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi
Teladorsagia circumcincta