EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.3.1 | HgCl2 | 0.1 mM, 100% inhibition of D-ribulose-5-phosphate 3-epimerase activity | Bos taurus | |
5.1.3.1 | potassium phosphate | 10 mM, 49% inhibition of D-ribulose-5-phosphate 3-epimerase activity | Bos taurus | |
5.1.3.1 | Sodium sulfate | 20 mM, 65% inhibition of D-ribulose-5-phosphate 3-epimerase activity | Bos taurus | |
5.3.1.34 | HgCl2 | 0.1 mM, 100% inhibition of D-erythrose 4-phosphate isomerase activity | Bos taurus | |
5.3.1.34 | pentulose 5-phosphate | i.e. enzymatic equilibrium mixture of D-ribulose 5-phosphate and D-xylulose 5-phosphate, 1 mM, 63% inhibition | Bos taurus | |
5.3.1.34 | potassium phosphate | 10 mM, 45% inhibition of D-erythrose 4-phosphate isomerase activity | Bos taurus | |
5.3.1.34 | Sodium sulfate | 20 mM, 66% inhibition of D-erythrose 4-phosphate isomerase activity | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.3.1 | 22500 | - |
2 * 22500, SDS-PAGE | Bos taurus |
5.1.3.1 | 43000 | - |
gel filtration | Bos taurus |
5.3.1.34 | 22500 | - |
2 * 22500, SDS-PAGE | Bos taurus |
5.3.1.34 | 43000 | - |
gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.1 | Bos taurus | - |
- |
- |
5.3.1.34 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.1 | - |
Bos taurus |
5.3.1.34 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.1.3.1 | liver | - |
Bos taurus | - |
5.3.1.34 | liver | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.3.1 | 9.05 | - |
substrate D-erythrose 4-phosphate, pH 7.0, 30°C | Bos taurus |
5.1.3.1 | 6570 | - |
substrate D-ribulose-5-phosphate, pH 7.0, 30°C | Bos taurus |
5.3.1.34 | 9.05 | - |
substrate D-erythrose 4-phosphate, pH 7.0, 30°C | Bos taurus |
5.3.1.34 | 6570 | - |
substrate D-ribulose-5-phosphate, pH 7.0, 30°C | Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
5.1.3.1 | 4°C, 10 mM Bistris/HCl buffer (pH 7.0), stable for about six months without any loss of enzymatic activity | Bos taurus |
5.3.1.34 | 4°C, 10 mM Bistris/HCl buffer (pH 7.0), stable for about six months without any loss of enzymatic activity | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.1 | D-erythrose 4-phosphate | reaction of EC 5.1.3.1 | Bos taurus | D-erythrulose 4-phosphate | - |
? | |
5.1.3.1 | D-ribulose-5-phosphate | - |
Bos taurus | D-xylulose 5-phosphate | - |
? | |
5.3.1.34 | D-erythrose 4-phosphate | - |
Bos taurus | D-erythrulose 4-phosphate | initially D-threose 4-phosphate is also formed. After 240 min, about 90% of tetrose 4-phosphate is D-erythrulose 4-phosphate, 5% is D-threose 4-phosphate | ? | |
5.3.1.34 | D-ribulose-5-phosphate | - |
Bos taurus | D-xylulose 5-phosphate | strong D-ribulose-5-phosphate 3-epimerase activity, reaction of EC 5.1.3.1 | ? | |
5.3.1.34 | D-threose 4-phosphate | 130% of the activity with D-erythrose 4-phosphate | Bos taurus | D-erythrulose 4-phosphate | D-threose 4-phosphate isomerizes non-enzymatically more easily than D-erythrose 4-phosphate to D-erythrulose 4-phosphate | ? | |
5.3.1.34 | additional information | enzyme can catalyze simultaneously both the isomerization and epimerization of D-erythrose 4-phosphate to D-erythrulose 4-phosphate and D-threose 4-phosphate. No isomerase activity with D-glyceraldehyde 3-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate and D-fructose 6-phosphate | Bos taurus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.3.1 | dimer | 2 * 22500, SDS-PAGE | Bos taurus |
5.3.1.34 | dimer | 2 * 22500, SDS-PAGE | Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.1 | 65 | - |
10 min, stable | Bos taurus |
5.3.1.34 | 65 | - |
10 min, stable | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.1 | 8.5 | - |
- |
Bos taurus |
5.3.1.34 | 8.5 | - |
- |
Bos taurus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.1 | 6.5 | 9.5 | 22 h, 0°C, stable | Bos taurus |
5.3.1.34 | 6.5 | 9.5 | 22 h, 0°C, stable | Bos taurus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.1.3.1 | Bos taurus | isoelectric focusing | - |
4.8 |
5.3.1.34 | Bos taurus | isoelectric focusing | - |
4.8 |