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Literature summary extracted from

  • Sikarwar, J.; Kaushik, S.; Sinha, M.; Kaur, P.; Sharma, S.; Singh, T.P.
    Cloning, expression, and purification of nucleoside diphosphate kinase from Acinetobacter baumannii (2013), Enzyme Res., 2013, 597028.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 gene ndk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 Urea complete enzyme inactivation at 6 M, 50% inhibition at 5 M Acinetobacter baumannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+ required Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + nucleoside diphosphate Acinetobacter baumannii
-
 ADP + nucleoside triphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Acinetobacter baumannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + nucleoside diphosphate
-
 Acinetobacter baumannii ADP + nucleoside triphosphate
-
 r
2.7.4.6 ATP + nucleoside diphosphate analysis of ATP binding by the enzyme at different concentrations and pH values, overview Acinetobacter baumannii ADP + nucleoside triphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDK
-
 Acinetobacter baumannii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Acinetobacter baumannii

General Information

EC Number General Information Comment Organism
2.7.4.6 physiological function nucleoside diphosphate kinase is involved in the catalysis of the transfer of gamma-phosphate from nucleoside triphosphate to nucleoside diphosphate and thus maintains the nucleotide pool. The dNTPs that are formed are used as precursors for DNA and RNA synthesis. NTPs, specifically GTP, are important for cellular macromolecular synthesis and signalling mechanisms. Ndk plays a pivotal role in crucial events like bacterial growth, signal transduction, and pathogenicity Acinetobacter baumannii