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Literature summary extracted from

  • Mathew, S.; Nadarajan, S.P.; Chung, T.; Park, H.H.; Yun, H.
    Biochemical characterization of thermostable omega-transaminase from Sphaerobacter thermophilus and its application for producing aromatic beta- and gamma-amino acids (2016), Enzyme Microb. Technol., 87-88, 52-60.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.18 expression in Escherichia coli Sphaerobacter thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.6.1.18 DMSO 20%, 43% residual activity after 12 h Sphaerobacter thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.18 Sphaerobacter thermophilus D1C218
-
-
2.6.1.18 Sphaerobacter thermophilus DSM 20745 D1C218
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.18 3.3
-
 substrate rac-beta-phenylalanine, 37°C, pH 8.0 Sphaerobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.18 3-amino-3-(3,4-dimethoxyphenyl)propanoate + pyruvate about 95% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid Sphaerobacter thermophilus 3-oxo-3-(3,4-dimethoxyphenyl)propanoate + L-alanine
-
 ?
2.6.1.18 3-amino-3-(3,4-dimethoxyphenyl)propanoate + pyruvate about 95% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid Sphaerobacter thermophilus DSM 20745 3-oxo-3-(3,4-dimethoxyphenyl)propanoate + L-alanine
-
 ?
2.6.1.18 3-amino-3-(4-fluorophenyl)propanoate + pyruvate about 80% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid Sphaerobacter thermophilus 3-oxo-3-(4-fluorophenyl)propanoate + L-alanine
-
 ?
2.6.1.18 3-amino-3-(4-fluorophenyl)propanoate + pyruvate about 80% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid Sphaerobacter thermophilus DSM 20745 3-oxo-3-(4-fluorophenyl)propanoate + L-alanine
-
 ?
2.6.1.18 3-amino-5-methylhexanoate + 2-oxohexanoate
-
 Sphaerobacter thermophilus 3-oxo-5-methylhexanoate + 2-aminohexanoate about 65% of the amine acceptor activity of pyruvate ?
2.6.1.18 3-amino-5-methylhexanoate + 2-oxohexanoate
-
 Sphaerobacter thermophilus DSM 20745 3-oxo-5-methylhexanoate + 2-aminohexanoate about 65% of the amine acceptor activity of pyruvate ?
2.6.1.18 3-amino-5-methylhexanoate + ethyl 2-oxopropanoate
-
 Sphaerobacter thermophilus 3-oxo-5-methylhexanoate + ethyl 2-aminopropanoate about 70% of the amine acceptor activity of pyruvate ?
2.6.1.18 3-amino-5-methylhexanoate + ethyl 2-oxopropanoate
-
 Sphaerobacter thermophilus DSM 20745 3-oxo-5-methylhexanoate + ethyl 2-aminopropanoate about 70% of the amine acceptor activity of pyruvate ?
2.6.1.18 3-amino-5-methylhexanoate + pyruvate about 85% of the amine donor activity of 4-amino-4-(4-methylphenyl)butanoic acid Sphaerobacter thermophilus 3-oxo-5-methylhexanoate + L-alanine
-
 ?
2.6.1.18 4-amino-4-(4-methylphenyl)butanoic acid + pyruvate
-
 Sphaerobacter thermophilus 4-oxo-4-(4-methylphenyl)butanoate + L-alanine
-
 ?
2.6.1.18 additional information enzyme has activity towards abroad range of substrates including amines, beta- and gamma-amino acids Sphaerobacter thermophilus ?
-
 ?
2.6.1.18 additional information enzyme has activity towards abroad range of substrates including amines, beta- and gamma-amino acids Sphaerobacter thermophilus DSM 20745 ?
-
 ?
2.6.1.18 rac-beta-phenylalanine + pyruvate
-
 Sphaerobacter thermophilus 3-oxo-3-phenylpropanoate + L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.18 Sthe_0848
-
 Sphaerobacter thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.18 60
-
 stable for at least 1 h Sphaerobacter thermophilus