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Literature summary extracted from

  • Yang, X.Q.; Zhang, Y.L.
    Characterization of glutathione S-transferases from Sus scrofa, Cydia pomonella and Triticum aestivum: their responses to cantharidin (2015), Enzyme Microb. Technol., 69, 1-9.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.18 cantharidin cantharidin significantly stimulates isoform GSTf3 activity Triticum aestivum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Sus scrofa
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Triticum aestivum
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Cydia pomonella

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 cantharidic acid
-
 Cydia pomonella
2.5.1.18 cantharidic acid
-
 Sus scrofa
2.5.1.18 cantharidic acid
-
 Triticum aestivum
2.5.1.18 cantharidin
-
 Cydia pomonella
2.5.1.18 additional information cantharidin has no effect on isoform SGSTalpha1 activity Sus scrofa
2.5.1.18 S-hexylglutathione
-
 Cydia pomonella
2.5.1.18 S-hexylglutathione
-
 Sus scrofa
2.5.1.18 S-hexylglutathione
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.091
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
2.5.1.18 0.2
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 0.27
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella
2.5.1.18 0.36
-
 glutathione at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 0.79
-
 glutathione at pH 7.5 and 30°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 24190
-
-
 Cydia pomonella
2.5.1.18 24490
-
-
 Triticum aestivum
2.5.1.18 25280
-
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Cydia pomonella
-
-
-
2.5.1.18 Sus scrofa
-
-
-
2.5.1.18 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni2+-NTA agarose column chromatography Sus scrofa
2.5.1.18 Ni2+-NTA agarose column chromatography Triticum aestivum
2.5.1.18 Ni2+-NTA agarose column chromatography Cydia pomonella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Sus scrofa S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Triticum aestivum S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Cydia pomonella S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 24190, calculated from amino acid sequence Cydia pomonella
2.5.1.18 ? x * 24490, calculated from amino acid sequence Triticum aestivum
2.5.1.18 ? x * 25280, calculated from amino acid sequence Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Sus scrofa
2.5.1.18 glutathione S-transferase
-
 Triticum aestivum
2.5.1.18 glutathione S-transferase
-
 Cydia pomonella
2.5.1.18 GSTalpha1
-
 Sus scrofa
2.5.1.18 GSTD1
-
 Cydia pomonella
2.5.1.18 GSTF3
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
-
 Sus scrofa
2.5.1.18 30
-
-
 Triticum aestivum
2.5.1.18 30
-
-
 Cydia pomonella

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 20 40 more than 50% activity between 20 and 40°C Sus scrofa
2.5.1.18 20 40 more than 50% activity between 20 and 40°C Triticum aestivum
2.5.1.18 20 40 more than 50% activity between 20 and 40°C Cydia pomonella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 10.9
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 16.4
-
 glutathione at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 99
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
2.5.1.18 141.7
-
 glutathione at pH 7.5 and 30°C Sus scrofa
2.5.1.18 610
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7 7.5 at 30°C Sus scrofa
2.5.1.18 7 7.5 at 30°C Triticum aestivum
2.5.1.18 7 7.5 at 30°C Cydia pomonella

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 8 more than 50% activity between pH 6.0 and 8.0 Sus scrofa
2.5.1.18 6 8 more than 50% activity between pH 6.0 and 8.0 Triticum aestivum
2.5.1.18 6 8 more than 50% activity between pH 6.0 and 8.0 Cydia pomonella

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Triticum aestivum calculated from amino acid sequence
-
 5.3
2.5.1.18 Cydia pomonella calculated from amino acid sequence
-
 6.8
2.5.1.18 Sus scrofa calculated from amino acid sequence
-
 8.9

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 55
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 91
-
 glutathione at pH 7.5 and 30°C Triticum aestivum
2.5.1.18 109
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
2.5.1.18 178
-
 glutathione at pH 7.5 and 30°C Sus scrofa
2.5.1.18 2283
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella