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Literature summary extracted from

  • Lin, S.F.; Chiou, C.M.; Tsai, Y.C.
    Purification and characterization of a glycerol oxidase from Penicillium sp. TS-622 (1996), Enzyme Microb. Technol., 18, 383-387.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.B4 Chloramine T 60% inhibition at 1 mM Penicillium sp.
1.1.3.B4 Hg2+ 60% inhibition at 1 mM Penicillium sp.
1.1.3.B4 hydroxylamine 87% inhibition at 1 mM Penicillium sp.
1.1.3.B4 KCN complete inhibition at 1 mM Penicillium sp.
1.1.3.B4 additional information EDTA, phenylglycoxyl, iodoacetic acid, and o-phenanthroline do not show significant inhibitory effects on the enzyme activity Penicillium sp.
1.1.3.B4 NaN3 complete inhibition at 1 mM Penicillium sp.
1.1.3.B4 Tetranitromethane 46% inhibition at 1 mM Penicillium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.B4 cell surface
-
 Penicillium sp. 9986
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.B4 Cu2+ 15% increase of activity in the presence of 1 mM Cu2+ Penicillium sp.
1.1.3.B4 Fe3+ 10% increase of activity in the presence of 1 mM Fe3+ Penicillium sp.
1.1.3.B4 additional information not influenced by Co2+, Mn2+, Mg2+, Pb2+, Ca2+, and Cd2+ Penicillium sp.
1.1.3.B4 Zn2+ 15% increase of activity in the presence of 1 mM Zn2+ Penicillium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.B4 400000
-
 gel filtration Penicillium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.B4 Penicillium sp.
-
-
-
1.1.3.B4 Penicillium sp. TS-622
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.B4 ammonium sulfate fractionation, Sephadex G-100 gel filtration, Fractogel DEAE-650M column chromatography, Toyopearl phenyl-650M column chromatography, and Ultrogel hydroxyapatite column chromatography Penicillium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.B4 mycelium
-
 Penicillium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.B4 1.7
-
 crude extract, at pH 7.0 and 30°C Penicillium sp.
1.1.3.B4 66.7
-
 after 39.2fold purification, at pH 7.0 and 30°C Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.B4 dihydroxyacetone + O2 132% activity compared to glycerol Penicillium sp. 3-hydroxy-2-oxopropanal + H2O2
-
 ?
1.1.3.B4 dihydroxyacetone + O2 132% activity compared to glycerol Penicillium sp. TS-622 3-hydroxy-2-oxopropanal + H2O2
-
 ?
1.1.3.B4 glycerol + O2 100% activity Penicillium sp. glyceraldehyde + H2O2
-
 ?
1.1.3.B4 glycerol + O2 100% activity Penicillium sp. TS-622 glyceraldehyde + H2O2
-
 ?
1.1.3.B4 additional information the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol Penicillium sp. ?
-
 ?
1.1.3.B4 additional information the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol Penicillium sp. TS-622 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.B4 45
-
-
 Penicillium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.B4 20 60 after 1 h of incubation, the enzyme is relatively stable from 20 to 40°C and loses most of its activity over 50°C Penicillium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.B4 6.5 7
-
 Penicillium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.B4 5.5 6.5 the enzyme is stable in the pH range from 5.5 to 6.5 Penicillium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.B4 heme
-
 Penicillium sp.