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Literature summary extracted from

  • Schuehle, K.; Nies, J.; Heider, J.
    An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin (2016), Environ. Microbiol., 18, 3120-3132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.15 expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.15 40000
-
-
 Aromatoleum aromaticum

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.15 Aromatoleum aromaticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.15 strep-tactin affinity column chromatography Aromatoleum aromaticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.15 0.024
-
 with (R/S)-phenylsuccinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.085
-
 with succinyl-CoA and (S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.103
-
 with succinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.108
-
 with (R/S)-phenylsuccinyl-CoA and (R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.122
-
 with succinyl-CoA and (R)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.136
-
 with succinyl-CoA and 2-oxo-indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.188
-
 with succinyl-CoA and indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.15 0.235
-
 with succinyl-CoA and(R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.15 (R/S)-phenylsuccinyl-CoA + (R,S)-benzylsuccinate 10% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum (R/S)-phenylsuccinate + (R,S)-benzylsuccinyl-CoA
-
 ?
2.8.3.15 (R/S)-phenylsuccinyl-CoA + (R,S)-phenylsuccinate 46% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum (R/S)-phenylsuccinate + (R,S)-phenylsuccinyl-CoA
-
 ?
2.8.3.15 additional information the enzyme shows no activity with benzylmalonate, acetyl-CoA, and cinnamate Aromatoleum aromaticum ?
-
 ?
2.8.3.15 succinyl-CoA + (R)-benzylsuccinate 52% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (R)-benzylsuccinyl-CoA
-
 ?
2.8.3.15 succinyl-CoA + (R,S)-benzylsuccinate 44% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (R,S)-benzylsuccinyl-CoA
-
 ?
2.8.3.15 succinyl-CoA + (R,S)-phenylsuccinate 100% activity Aromatoleum aromaticum succinate + 2-phenylsuccinyl-CoA + 3-phenylsuccinyl-CoA
-
 ?
2.8.3.15 succinyl-CoA + (S)-benzylsuccinate 36% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + (S)-benzylsuccinyl-CoA
-
 ?
2.8.3.15 succinyl-CoA + 2-oxoindole-3-acetate 58% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + 2-oxoindole-3-acetyl-CoA
-
 ?
2.8.3.15 succinyl-CoA + indole-3-acetate 80% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate Aromatoleum aromaticum succinate + indole-3-acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.15 ? x * 40000, Strep-tagged enzyme, SDS-PAGE Aromatoleum aromaticum

Synonyms

EC Number Synonyms Comment Organism
2.8.3.15 IaaL
-
 Aromatoleum aromaticum
2.8.3.15 phenylsuccinyl-CoA transferase
-
 Aromatoleum aromaticum
2.8.3.15 succinyl-CoA:phenylsuccinate CoA-transferase
-
 Aromatoleum aromaticum