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Literature summary extracted from

  • Fraga, D.; Aryal, M.; Hall, J.E.; Rae, E.; Snider, M.
    Characterization of the arginine kinase isoforms in Caenorhabditis elegans (2015), Comp. Biochem. Physiol. B, 187, 85-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 gene argk-1, DNA and amino acid sequence determination and analysis, sequence comparisons, molecular genetic and phylogenetic analysis Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 additional information
-
 additional information steady-state kinetic analysis, sequential ternary kinetic model, overview Caenorhabditis elegans
2.7.3.3 0.023
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.054
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.16
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.35
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.37
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.41
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 0.88
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 1.26
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.3 ATP + L-arginine Caenorhabditis elegans
-
 ADP + Nomega-phospho-L-arginine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Caenorhabditis elegans O01854 gene argk-3 or F32B5.1
-
2.7.3.3 Caenorhabditis elegans O45011 gene argk-2 or W10C8.5
-
2.7.3.3 Caenorhabditis elegans O45518 gene argk-1 or F44G3.2
-
2.7.3.3 Caenorhabditis elegans Q10454 gene argk-4 or F46H5.3
-
2.7.3.3 Caenorhabditis elegans Q27535 gene argk-5 or ZC434.8
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-arginine
-
 Caenorhabditis elegans ADP + Nomega-phospho-L-arginine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 ARGK-2
-
 Caenorhabditis elegans
2.7.3.3 F32B5.1
-
 Caenorhabditis elegans
2.7.3.3 F44G3.2
-
 Caenorhabditis elegans
2.7.3.3 F46H5.3
-
 Caenorhabditis elegans
2.7.3.3 W10C8.5
-
 Caenorhabditis elegans
2.7.3.3 ZC434.8
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.3 25
-
 assay at Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.3 11.4
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 11.4
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 40
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 40
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 45
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 45
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 180
-
 ATP pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 180
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 6
-
 reverse direction assay at Caenorhabditis elegans
2.7.3.3 8
-
 assay at Caenorhabditis elegans
2.7.3.3 8
-
 forward direction assay at Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.7.3.3 evolution the enzyme belongs to the family of phosphagen kinases, molecular genetic and phylogenetic analysis, overview. The gene family has undergone extensive intron loss and gain within the suborder Rhabditina. Caenorhabditis elegans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.3.3 31
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 45
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 440
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans
2.7.3.3 900
-
 L-arginine pH 8.0, 25°C, recombinant enzyme Caenorhabditis elegans