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Literature summary extracted from

  • Palmer, A.; Begres, B.N.; Van Houten, J.M.; Snider, M.J.; Fraga, D.
    Characterization of a putative oomycete taurocyamine kinase: implications for the evolution of the phosphagen kinase family (2013), Comp. Biochem. Physiol. B, 166, 173-181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.4 gene PHYSODRAFT_566100, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminal His6-PsPK protein in Escherichia coli strain Rosetta 2 (DE3) Phytophthora sojae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.4 additional information
-
 additional information steady-state kinetic analysis, overview. Analysis of quaternary structure and cooperativity in ligand-binding by ITC: absence of inter-subunit cooperativity in forming the PsTK–TSAC Phytophthora sojae
2.7.3.4 0.52
-
 Taurocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae
2.7.3.4 1.8
-
 ATP recombinant enzyme, pH 9.0, 30°C, with taurocyamine Phytophthora sojae
2.7.3.4 4.6
-
 ATP recombinant enzyme, pH 9.0, 30°C, with glycocyamine Phytophthora sojae
2.7.3.4 6.1
-
 glycocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.4 Mg2+ required Phytophthora sojae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.4 ATP + glycocyamine Phytophthora sojae low activity ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + glycocyamine Phytophthora sojae P6497 low activity ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + taurocyamine Phytophthora sojae
-
 ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine Phytophthora sojae P6497
-
 ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 additional information Phytophthora sojae the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview ?
-
 ?
2.7.3.4 additional information Phytophthora sojae P6497 the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.4 Phytophthora sojae G5ADV1 gene PHYSODRAFT_566100
-
2.7.3.4 Phytophthora sojae P6497 G5ADV1 gene PHYSODRAFT_566100
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.4 recombinant N-terminal His6-PsPK protein from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration Phytophthora sojae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.4 ATP + glycocyamine low activity Phytophthora sojae ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + glycocyamine low activity Phytophthora sojae P6497 ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + taurocyamine
-
 Phytophthora sojae ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine
-
 Phytophthora sojae P6497 ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 additional information the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview Phytophthora sojae ?
-
 ?
2.7.3.4 additional information no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine Phytophthora sojae ?
-
 ?
2.7.3.4 additional information the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview Phytophthora sojae P6497 ?
-
 ?
2.7.3.4 additional information no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine Phytophthora sojae P6497 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.4 dimer 2 + 51000, about, sequence calculation, the enzyme is dimeric but lacks cooperativity between the subunits in forming a transition state analogue complex Phytophthora sojae

Synonyms

EC Number Synonyms Comment Organism
2.7.3.4 hypotaurocyamine kinase
-
 Phytophthora sojae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.4 30
-
 assay at Phytophthora sojae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.4 1.4
-
 glycocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae
2.7.3.4 94.1
-
 Taurocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.4 9
-
 assay at Phytophthora sojae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.4 ATP
-
 Phytophthora sojae

General Information

EC Number General Information Comment Organism
2.7.3.4 evolution the enzyme belongs to the phosphagen kinases family of enzymes. Phylogenetic analysis of oomycete enzymes, overview Phytophthora sojae
2.7.3.4 physiological function within animal species, these enzymes play a critical role in energy homeostasis by catalyzing the reversible transfer of a high-energy phosphoryl group from Mg-ATP to an acceptor molecule containing a guanidinium group Phytophthora sojae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.3.4 0.23
-
 glycocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae
2.7.3.4 180
-
 Taurocyamine recombinant enzyme, pH 9.0, 30°C Phytophthora sojae