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Literature summary extracted from

  • Ayuso-Fernandez, I.; Galmes, M.; Bastida, A.; Garcia-Junceda, E.
    Aryl sulfotransferase from Haliangium ochraceum: A versatile tool for the sulfation of small molecules (2014), ChemCatChem, 6, 1059-1065.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 expressed in Escherichia coli BL21(DE3) cells Haliangium ochraceum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.004
-
 3'-phosphoadenylyl sulfate at pH 7.0 and 25°C Haliangium ochraceum
2.8.2.1 0.0459
-
 4-nitrophenyl sulfate at pH 7.0 and 25°C Haliangium ochraceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 40000
-
 x * 40000, SDS-PAGE Haliangium ochraceum

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Haliangium ochraceum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 Ni2+-IDA agarose column chromatography Haliangium ochraceum

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 4°C, the enzyme retains over 90% of its activity for several days Haliangium ochraceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 4,4'-biphenol best substrate with 93% activity Haliangium ochraceum adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenyl sulfate
-
 Haliangium ochraceum 4-nitrophenol + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + catechol
-
 Haliangium ochraceum adenosine 3',5'-bisphosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.1 ? x * 40000, SDS-PAGE Haliangium ochraceum
2.8.2.1 ? x * 37221, calculated from amino acid sequence Haliangium ochraceum

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 ArylST
-
 Haliangium ochraceum
2.8.2.1 AST
-
 Haliangium ochraceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.1 25 45 there is a significant decrease of the half-life of the enzyme between 25 and 37°C. At 25°C the half-life is over 3 h, while at 37°C it is 36-37 min, and at 45°C less than 6 min Haliangium ochraceum