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Literature summary extracted from

  • Minagawa, K.; Zhang, Y.; Ito, T.; Bai, L.; Deng, Z.; Mahmud, T.
    ValC, a new type of C7-Cyclitol kinase involved in the biosynthesis of the antifungal agent validamycin A (2007), ChemBioChem, 8, 632-641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.214 expression in Escherichia coli Streptomyces hygroscopicus subsp. jinggangensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.214 0.019
-
 valienone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis
2.7.1.214 0.026
-
 validone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.214 36750
-
-
 Streptomyces hygroscopicus subsp. jinggangensis
2.7.1.214 40000
-
-
 Streptomyces hygroscopicus subsp. jinggangensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.214 ATP + validone Streptomyces hygroscopicus subsp. jinggangensis
-
 ADP + validone 7-phosphate
-
 ?
2.7.1.214 ATP + validone Streptomyces hygroscopicus subsp. jinggangensis 5008
-
 ADP + validone 7-phosphate
-
 ?
2.7.1.214 ATP + valienone Streptomyces hygroscopicus subsp. jinggangensis
-
 ADP + valienone 7-phosphate
-
 ?
2.7.1.214 ATP + valienone Streptomyces hygroscopicus subsp. jinggangensis 5008
-
 ADP + valienone 7-phosphate
-
 ?
2.7.1.214 additional information Streptomyces hygroscopicus subsp. jinggangensis ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives ?
-
 ?
2.7.1.214 additional information Streptomyces hygroscopicus subsp. jinggangensis 5008 ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.214 Streptomyces hygroscopicus subsp. jinggangensis Q3T6E2
-
-
2.7.1.214 Streptomyces hygroscopicus subsp. jinggangensis 5008 Q3T6E2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.214 ATP + validone
-
 Streptomyces hygroscopicus subsp. jinggangensis ADP + validone 7-phosphate
-
 ?
2.7.1.214 ATP + validone
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 ADP + validone 7-phosphate
-
 ?
2.7.1.214 ATP + valienone
-
 Streptomyces hygroscopicus subsp. jinggangensis ADP + valienone 7-phosphate
-
 ?
2.7.1.214 ATP + valienone
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 ADP + valienone 7-phosphate
-
 ?
2.7.1.214 additional information ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives Streptomyces hygroscopicus subsp. jinggangensis ?
-
 ?
2.7.1.214 additional information ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives Streptomyces hygroscopicus subsp. jinggangensis 5008 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.214 ? x * 40000, SDS-PAGE of recombinant His-tagged protein, x * 36750, calculated Streptomyces hygroscopicus subsp. jinggangensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.214 valC
-
 Streptomyces hygroscopicus subsp. jinggangensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.214 28
-
 assay at Streptomyces hygroscopicus subsp. jinggangensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.214 3.5
-
 valienone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis
2.7.1.214 7.3
-
 validone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.214 7.6
-
 assay at Streptomyces hygroscopicus subsp. jinggangensis

General Information

EC Number General Information Comment Organism
2.7.1.214 physiological function inactivation of valC results in mutants that lack the ability to produce validamycin A Streptomyces hygroscopicus subsp. jinggangensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.214 180
-
 valienone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis
2.7.1.214 286
-
 validone pH 7.6, 28°C Streptomyces hygroscopicus subsp. jinggangensis