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Literature summary extracted from

  • Bulut, D.; Duangdee, N.; Groeger, H.; Berkessel, A.; Hummel, W.
    Screening, molecular cloning, and biochemical characterization of an alcohol dehydrogenase from Pichia pastoris useful for the kinetic resolution of a racemic beta-hydroxy-beta-trifluoromethyl ketone (2016), ChemBioChem, 17, 1349-1358.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 expression in Escherichia coli Komagataella phaffii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.219 additional information fusion of ADH to the N-terminal His-tag causes a strong improvement of about 95% in activity Komagataella phaffii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.219 0.0161
-
NADPH pH 7.0, 25°C Komagataella phaffii
1.1.1.219 1.15
-
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one pH 7.0, 25°C Komagataella phaffii
1.1.1.219 5.2
-
benzaldehyde pH 7.0, 25°C Komagataella phaffii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.219 41000
-
-
Komagataella phaffii
1.1.1.219 41400
-
-
Komagataella phaffii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Komagataella phaffii C4R4L0
-
-
1.1.1.219 Komagataella phaffii GS115 C4R4L0
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.219 4°C, 50% loss of activity in 100 days Komagataella phaffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (2E)-hex-2-enal + NADPH + H+ 97% of the activity with benzaldehyde Komagataella phaffii (2E)-hex-2-en-1-ol + NADP+
-
?
1.1.1.219 (2E)-hex-2-enal + NADPH + H+ 97% of the activity with benzaldehyde Komagataella phaffii GS115 (2E)-hex-2-en-1-ol + NADP+
-
?
1.1.1.219 (2S)-hexan-2-ol + NADP+
-
Komagataella phaffii hexan-2-one + NADPH + H+
-
r
1.1.1.219 (2S)-hexan-2-ol + NADP+
-
Komagataella phaffii GS115 hexan-2-one + NADPH + H+
-
r
1.1.1.219 (4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one + NADPH + H+ enzyme specifically reduces the S-enantiomer Komagataella phaffii (2S)-1,1,1-trifluoro-2-phenylpentane-2,4-diol + NADP+
-
?
1.1.1.219 2-methylpentanal + NADPH + H+ 249% of the activity with benzaldehyde Komagataella phaffii 2-methyl-pentan-1-ol + NADP+
-
?
1.1.1.219 benzaldehyde + NADPH + H+
-
Komagataella phaffii benzyl alcohol + NADP+
-
?
1.1.1.219 benzaldehyde + NADPH + H+
-
Komagataella phaffii GS115 benzyl alcohol + NADP+
-
?
1.1.1.219 butanal + NADPH + H+ 176% of the activity with benzaldehyde Komagataella phaffii butan-1-ol + NADP+
-
?
1.1.1.219 butanal + NADPH + H+ 176% of the activity with benzaldehyde Komagataella phaffii GS115 butan-1-ol + NADP+
-
?
1.1.1.219 heptan-2-one + NADPH + H+
-
Komagataella phaffii (2S)-heptan-2-ol + NADP+ 99% conversion, 99% enantiomeric excess r
1.1.1.219 hexan-2-one + NADPH + H+
-
Komagataella phaffii (2S)-hexan-2-ol + NADP+ 99% conversion, 99% enantiomeric excess r
1.1.1.219 hexanal + NADPH + H+ 74% of the activity with benzaldehyde Komagataella phaffii hexan-1-ol + NADP+
-
?
1.1.1.219 additional information linear and branched aliphatic aldehydes are good substrates Komagataella phaffii ?
-
?
1.1.1.219 additional information linear and branched aliphatic aldehydes are good substrates Komagataella phaffii GS115 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.219 ? x * 41000, SDS-PAGE, x * 41400, calculated Komagataella phaffii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 PAS_chr3_0449
-
Komagataella phaffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 40 45
-
Komagataella phaffii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.219 50
-
5 min, rapid inactivation Komagataella phaffii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.219 44.5
-
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one pH 7.0, 25°C Komagataella phaffii
1.1.1.219 54.4
-
benzaldehyde pH 7.0, 25°C Komagataella phaffii
1.1.1.219 58.4
-
NADPH pH 7.0, 25°C Komagataella phaffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 5.5
-
-
Komagataella phaffii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 4 8
-
Komagataella phaffii