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Literature summary extracted from

  • Spickermann, D.; Hausmann, S.; Degering, C.; Schwaneberg, U.; Leggewie, C.
    Engineering of highly selective variants of Parvibaculum lavamentivorans alcohol dehydrogenase (2014), ChemBioChem, 15, 2050-2052.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1
-
 Parvibaculum lavamentivorans
1.1.1.B52
-
 Parvibaculum lavamentivorans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B52 A158A/N162P 96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion Parvibaculum lavamentivorans
1.1.1.B52 A158D/N162G 96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion Parvibaculum lavamentivorans
1.1.1.B52 A158D/N162Q 95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion Parvibaculum lavamentivorans
1.1.1.B52 A158H/N162G/K202Q/L224W mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5% Parvibaculum lavamentivorans
1.1.1.B52 A158H/N162P 98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion Parvibaculum lavamentivorans
1.1.1.B52 A158K/N162M 97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion Parvibaculum lavamentivorans
1.1.1.B52 K202N/L224M 89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion Parvibaculum lavamentivorans
1.1.1.B52 K202Q/L224W 95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion Parvibaculum lavamentivorans
1.1.1.B52 K202R/L224W 94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion Parvibaculum lavamentivorans
1.1.1.B52 K202S/L224Y 89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion Parvibaculum lavamentivorans
1.1.1.B52 N162A 93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion Parvibaculum lavamentivorans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Parvibaculum lavamentivorans
-
-
-
1.1.1.B52 Parvibaculum lavamentivorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 additional information the enzyme additionally catalyzes selective reduction of 3-quinuclidinone to (R)-3-quinuclidinol, with 84% ee and 62% conversion after 22 h Parvibaculum lavamentivorans ?
-
 ?
1.1.1.B52 3-quinuclidinone + NADH + H+
-
 Parvibaculum lavamentivorans (R)-3-quinuclidinol + NAD+ 84% ee, 62% conversion after 22 h ?
1.1.1.B52 additional information enzyme is an alcohol dehydrogenase, reaction of EC 1.1.1.1 Parvibaculum lavamentivorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B52 alcohol dehydrogenase
-
 Parvibaculum lavamentivorans