EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.127 | gene ITPKA, recombinnant expression of N-terminally GST-tagged isozyme ITPKA in Escherichia coli strain BL21 C41lys | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.127 | synapse | spine | Mus musculus | 45202 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.127 | Ca2+ | activates | Mus musculus | |
2.7.1.127 | Mg2+ | required | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.127 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | Mus musculus | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
2.7.1.127 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | Mus musculus C57BL/6 | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.127 | Mus musculus | Q8R071 | gene Itpka | - |
2.7.1.127 | Mus musculus C57BL/6 | Q8R071 | gene Itpka | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.127 | recombinant N-terminally GST-tagged isozyme ITPKA from Escherichia coli strain BL21 C41lys by glutathione affinity chromatography | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.127 | cervical spine | - |
Mus musculus | - |
2.7.1.127 | hippocampus | - |
Mus musculus | - |
2.7.1.127 | neuron | isozyme inositol-1,4,5-trisphosphate-3-kinase-A is a neuron-specific, actin bundling protein concentrated at dendritic spines | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.127 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Mus musculus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
2.7.1.127 | ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Mus musculus C57BL/6 | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.127 | inositol-1,4,5-trisphosphate-3-kinase-A | - |
Mus musculus |
2.7.1.127 | ITPKA | - |
Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.127 | 7.5 | - |
assay at | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.127 | ATP | - |
Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.127 | malfunction | ITPKA depletion in mice increases the number of hippocampal spine-synapses while reducing average spine length, depletion of ITPKA reduces the length of dendritic spines of hippocampal pyramidal cells | Mus musculus |
2.7.1.127 | physiological function | in addition to cross-linking actin filaments, ITPKA strongly inhibits Arp2/3-complex induced actin filament branching by displacing the complex from F-actin. n vivo ITPKA negatively regulates formation and/or maintenance of synaptic contacts in the mammalian brain. On the molecular level this effect appears to result from the ITPKA-mediated inhibition of Arp2/3-complex F-actin branching activity. ITPKA does not affect the F-actin bundling activity of drebrin A | Mus musculus |