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Literature summary extracted from

  • Yalcin, A.; Clem, B.F.; Imbert-Fernandez, Y.; Ozcan, S.C.; Peker, S.; ONeal, J.; Klarer, A.C.; Clem, A.L.; Telang, S.; Chesney, J.
    6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27 (2014), Cell Death Dis., 5, e1337.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 additional information siRNA silencing of endogenous PFKFB3 in HeLa cells by siRNA Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one the PFKFB3 inhibitor, 3PO, increases p27 protein in Lewis lung carcinoma cells in vitro and in vivo Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.105 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Homo sapiens
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Homo sapiens Q16875 gene PFKFB3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 HCT-116 cell PFKFB3 is overexpressed in human cancers Homo sapiens
-
2.7.1.105 HeLa cell
-
 Homo sapiens
-
2.7.1.105 HeLa cell PFKFB3 is overexpressed in human cancers Homo sapiens
-
2.7.1.105 Lewis lung carcinoma cell
-
 Homo sapiens
-
2.7.1.105 LLC cell PFKFB3 is overexpressed in human cancers Homo sapiens
-
2.7.1.105 additional information PFKFB3 is overexpressed in human cancers Homo sapiens
-
2.7.1.105 PANC-1 cell PFKFB3 is overexpressed in human cancers Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.105 6-phosphofructo-2-kinase
-
 Homo sapiens
2.7.1.105 PFKFB3
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.105 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.105 malfunction siRNA silencing of endogenous PFKFB3 inhibits Cdk1 activity, which in turn stabilizes p27 protein levels causing cell cycle arrest at G1/S and increased apoptosis in HeLa cells. PFKFB3 inhibition completely suppresses cell proliferation and results in increased early and late apoptotic cells. PFKFB3 inhibition results in increased nuclear and cytoplasmic p27 protein but has no effect on p57 or p21. Blockade of cell cycle progression and stimulation of apoptosis by PFKFB3 inhibition is mediated by p27 Homo sapiens
2.7.1.105 metabolism the family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) comprises well established regulators of glucose metabolism via their synthesis of fructose-2,6 bisphosphate, a potent allosteric activator of 6-phosphofructo-1-kinase. But PFKFB3 and fructose-2,6 bisphosphate function not only as regulators of Pfk-1 but also of Cdk1 activity, and therefore serve to couple glucose metabolism with cell proliferation and survival in transformed cells Homo sapiens
2.7.1.105 physiological function PFKFB3 is overexpressed in human cancers, regulated by HIF-1alpha, Akt and PTEN, and required for the survival and growth of multiple cancer types. Role of PFKFB3 in regulating Cdk1- and p27-mediated G1/S blockade and apoptosis Homo sapiens