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Literature summary extracted from

  • Berg, T.O.; Gurung, M.K.; Altermark, B.; Smalas, A.O.; Raeder, I.L.
    Characterization of the N-acetylneuraminic acid synthase (NeuB) from the psychrophilic fish pathogen Moritella viscosa (2015), Carbohydr. Res., 402, 133-145.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.56 synthesis the enzyme might be useful for production of sialic acids and derivates at low temperatures Moritella viscosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.56 gene MvNeuB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS Moritella viscosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.56 Co2+ stabilizes at low and inhibits at higher concentrations Moritella viscosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.56 additional information
-
 additional information Michaelis-Menten kinetics, overview Moritella viscosa
2.5.1.56 0.8
-
 phosphoenolpyruvate pH 7.3, 30°C, recombinant enzyme Moritella viscosa
2.5.1.56 18
-
 N-acetyl-D-mannosamine pH 7.3, 30°C, recombinant enzyme Moritella viscosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.56 Co2+ activates and stabilizes at low and inhibits at higher concentrations Moritella viscosa
2.5.1.56 Mg2+ requires Mn2+ or Mg2+, activates and stabilizes Moritella viscosa
2.5.1.56 Mn2+ requires Mn2+ or Mg2+, activates and stabilizes Moritella viscosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.56 38000
-
-
 Moritella viscosa
2.5.1.56 74000
-
 recombinant enzyme, gel filtration Moritella viscosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.56 phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O Moritella viscosa
-
 phosphate + N-acetylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Moritella viscosa A0A090IMH4 gene MvNeuB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.56 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration Moritella viscosa

Storage Stability

EC Number Storage Stability Organism
2.5.1.56 4°C, purified His6-tagged recombinant enzyme, stable for only 3-4 weeks, the enzyme readily aggregated at room temperature Moritella viscosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
 Moritella viscosa phosphate + N-acetylneuraminate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.56 homodimer 2 * 38935, sequence calculation, 2 * 38000, about, recombinant enzyme, SDS-PAGE Moritella viscosa
2.5.1.56 More structure comparisons Moritella viscosa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.56 N-acetylneuraminic acid synthase
-
 Moritella viscosa
2.5.1.56 NeuB
-
 Moritella viscosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.56 30
-
 recombinant enzyme Moritella viscosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.56 5 50 the relative enzyme activity is approximately 20% at 5°C, and increasing to a maximum at around 30°C, and decreases rapidly above 30°C, no activity remains at 55°C. The enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme Moritella viscosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.56 additional information
-
 the enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme Moritella viscosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.56 3.72
-
 N-acetyl-D-mannosamine pH 7.3, 30°C, recombinant enzyme Moritella viscosa
2.5.1.56 3.75
-
 phosphoenolpyruvate pH 7.3, 30°C, recombinant enzyme Moritella viscosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.56 7.3
-
 recombinant enzyme Moritella viscosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.56 7 10 activity range, inactive at pH 6.5, profile overview Moritella viscosa