Literature summary extracted from
Berg, T.O.; Gurung, M.K.; Altermark, B.; Smalas, A.O.; Raeder, I.L.
Characterization of the N-acetylneuraminic acid synthase (NeuB) from the psychrophilic fish pathogen Moritella viscosa (2015), Carbohydr. Res., 402, 133-145.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.5.1.56 |
synthesis |
the enzyme might be useful for production of sialic acids and derivates at low temperatures |
Moritella viscosa |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.56 |
gene MvNeuB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS |
Moritella viscosa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
Co2+ |
stabilizes at low and inhibits at higher concentrations |
Moritella viscosa |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
additional information |
- |
additional information |
Michaelis-Menten kinetics, overview |
Moritella viscosa |
|
2.5.1.56 |
0.8 |
- |
phosphoenolpyruvate |
pH 7.3, 30°C, recombinant enzyme |
Moritella viscosa |
|
2.5.1.56 |
18 |
- |
N-acetyl-D-mannosamine |
pH 7.3, 30°C, recombinant enzyme |
Moritella viscosa |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
Co2+ |
activates and stabilizes at low and inhibits at higher concentrations |
Moritella viscosa |
|
2.5.1.56 |
Mg2+ |
requires Mn2+ or Mg2+, activates and stabilizes |
Moritella viscosa |
|
2.5.1.56 |
Mn2+ |
requires Mn2+ or Mg2+, activates and stabilizes |
Moritella viscosa |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.5.1.56 |
38000 |
- |
- |
Moritella viscosa |
2.5.1.56 |
74000 |
- |
recombinant enzyme, gel filtration |
Moritella viscosa |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.5.1.56 |
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O |
Moritella viscosa |
- |
phosphate + N-acetylneuraminate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.56 |
Moritella viscosa |
A0A090IMH4 |
gene MvNeuB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.56 |
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration |
Moritella viscosa |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.5.1.56 |
4°C, purified His6-tagged recombinant enzyme, stable for only 3-4 weeks, the enzyme readily aggregated at room temperature |
Moritella viscosa |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.56 |
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O |
- |
Moritella viscosa |
phosphate + N-acetylneuraminate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.56 |
homodimer |
2 * 38935, sequence calculation, 2 * 38000, about, recombinant enzyme, SDS-PAGE |
Moritella viscosa |
2.5.1.56 |
More |
structure comparisons |
Moritella viscosa |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.56 |
N-acetylneuraminic acid synthase |
- |
Moritella viscosa |
2.5.1.56 |
NeuB |
- |
Moritella viscosa |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.5.1.56 |
30 |
- |
recombinant enzyme |
Moritella viscosa |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
2.5.1.56 |
5 |
50 |
the relative enzyme activity is approximately 20% at 5°C, and increasing to a maximum at around 30°C, and decreases rapidly above 30°C, no activity remains at 55°C. The enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme |
Moritella viscosa |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
2.5.1.56 |
additional information |
- |
the enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme |
Moritella viscosa |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
3.72 |
- |
N-acetyl-D-mannosamine |
pH 7.3, 30°C, recombinant enzyme |
Moritella viscosa |
|
2.5.1.56 |
3.75 |
- |
phosphoenolpyruvate |
pH 7.3, 30°C, recombinant enzyme |
Moritella viscosa |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.5.1.56 |
7.3 |
- |
recombinant enzyme |
Moritella viscosa |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.5.1.56 |
7 |
10 |
activity range, inactive at pH 6.5, profile overview |
Moritella viscosa |