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Literature summary extracted from

  • Smyth, K.M.; Marchant, A.
    Conservation of the 2-keto-3-deoxymanno-octulosonic acid (Kdo) biosynthesis pathway between plants and bacteria (2013), Carbohydr. Res., 380, 70-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 gene kdsA1, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli Arabidopsis thaliana
2.5.1.55 gene kdsA2, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 additional information gene kdsA1 single knockout and kdsA1/kdsA2 double knockout mutant constructions Arabidopsis thaliana
2.5.1.55 additional information gene kdsA2 single knockout and kdsA1/kdsA2 double knockout mutant constructions Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine PD404,182,displays strong inhibition in vitro, but is ineffective in vivo against Gram-negative bacteria due to an inability to cross the bacterial plasma membran Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Escherichia coli
-
 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Arabidopsis thaliana
-
 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Arabidopsis thaliana Q6NQL4 gene kdsA2; gene kdsA2
-
2.5.1.55 Arabidopsis thaliana Q9AV97 gene kdsA1; gene kdsA1
-
2.5.1.55 Escherichia coli
-
 gene kdsA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate the catalytic mechanism is thought to occur by correct substrate proximity and orientation through a series of sequential conformational changes in which phosphoenolpyruvate binding precedes that of D-arabinose 5-phosphate and the release of inorganic phosphate (Pi) precedes dissociation of the 3-deoxy-D-manno-octulosonate 8-phosphate product Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Arabidopsis thaliana 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 dimer in solution Arabidopsis thaliana
2.5.1.55 tetramer tetrameric quaternary structure with an active site in each monomer, by NMR study and crystal structure analysis Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 KDO-8-P synthase
-
 Escherichia coli
2.5.1.55 KDO-8-P synthase
-
 Arabidopsis thaliana
2.5.1.55 Kdo-8-phosphate synthase
-
 Escherichia coli
2.5.1.55 Kdo-8-phosphate synthase
-
 Arabidopsis thaliana
2.5.1.55 KDOPS
-
 Escherichia coli
2.5.1.55 KDOPS
-
 Arabidopsis thaliana
2.5.1.55 KdsA
-
 Arabidopsis thaliana
2.5.1.55 KdsA
-
 Escherichia coli
2.5.1.55 KdsA1
-
 Arabidopsis thaliana
2.5.1.55 KdsA2
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 5.9
-
 phosphoenolpyruvate pH and temperature not specified in the publication, recombinant enzyme Arabidopsis thaliana
2.5.1.55 5.9
-
 D-arabinose 5-phosphate pH and temperature not specified in the publication, recombinant enzyme Arabidopsis thaliana
2.5.1.55 6.8
-
 phosphoenolpyruvate pH and temperature not specified in the publication Escherichia coli
2.5.1.55 6.8
-
 D-arabinose 5-phosphate pH and temperature not specified in the publication Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.55 0.000026
-
 6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine pH and temperature not specified in the publication Escherichia coli

General Information

EC Number General Information Comment Organism
2.5.1.55 malfunction Arabidopsis thaliana kdsA male gametophyte with lethal phenotype Arabidopsis thaliana
2.5.1.55 malfunction while single T-DNA mutants of either gene display no visible phenotypes, generation of a double knockout mutant has not been possible due to a failure of haploid pollen tube elongation Arabidopsis thaliana