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Literature summary extracted from

  • Ken, C.; Chang, C.; Wen, L.; Huang, J.; Lin, C.
    Biochemical characterization of a functional recombinant aryl-alcohol dehydrogenase from Taiwanofungus camphorata (2014), Bot. Stud., 55, 14.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.91 expressed in Saccharomyces cerevisiae Taiwanofungus camphoratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.91 0.25
-
veratraldehyde at pH 6.0 temperature not specified in the publication Taiwanofungus camphoratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.91 39000
-
1 * 39000, SDS-PAGE Taiwanofungus camphoratus
1.1.1.91 40580
-
-
Taiwanofungus camphoratus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.91 Taiwanofungus camphoratus V9HZX4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.91 Ni-NTA column chromatography Taiwanofungus camphoratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.91 fruit body
-
Taiwanofungus camphoratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.91 2,4-dimethoxybenzyl alcohol + NADP+
-
Taiwanofungus camphoratus 2,4-dimethoxybenzyl aldehyde + NADPH + H+
-
?
1.1.1.91 3,4-dimethoxybenzyl alcohol + NADP+
-
Taiwanofungus camphoratus ?
-
?
1.1.1.91 3,4-dimethoxybenzyl alcohol + NADP+
-
Taiwanofungus camphoratus veratraldehyde + NADPH + H+
-
r
1.1.1.91 4-(hydroxymethyl)benzoic acid + NAD+
-
Taiwanofungus camphoratus ? + NADH + H+
-
?
1.1.1.91 benzaldehyde + NADPH + H+ reduction of benzaldehyde at pH 6.0 is NADPH-dependent while NADH does not yield detectable activity under the same conditions Taiwanofungus camphoratus benzyl alcohol + NADP+
-
r
1.1.1.91 benzyl alcohol + NAD+ oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent while NADP+ did not yield detectable activity under the same conditions Taiwanofungus camphoratus benzaldehyde + NADPH + H+
-
r
1.1.1.91 benzyl alcohol + NADP+
-
Taiwanofungus camphoratus benzaldehyde + NADPH + H+
-
?
1.1.1.91 veratraldehyde + NADPH + H+
-
Taiwanofungus camphoratus 3,4-dimethoxybenzyl alcohol + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.91 monomer 1 * 39000, SDS-PAGE Taiwanofungus camphoratus
1.1.1.91 monomer 1 * 40580, MALDI-TOF mass spectrometry Taiwanofungus camphoratus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.91 AAD
-
Taiwanofungus camphoratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.91 58
-
the enzyme's half-life of deactivation at 58°C is 4.2 min. There is no detectable enzyme activity when it is heated above 60°C Taiwanofungus camphoratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.91 6
-
with veratraldehyde as substrate Taiwanofungus camphoratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.91 4 10 at pH 8.0 and 10.0 the enzyme retains 62% activity, while the enzyme retains 13% activity at pH 4.0 Taiwanofungus camphoratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.91 NAD+ oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent Taiwanofungus camphoratus
1.1.1.91 NADP+
-
Taiwanofungus camphoratus
1.1.1.91 NADPH reduction of benzaldehyde at pH 6.0 is NADPH-dependent Taiwanofungus camphoratus