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Literature summary extracted from

  • Schlesier, J.; Siegrist, J.; Gerhardt, S.; Erb, A.; Blaesi, S.; Richter, M.; Einsle, O.; Andexer, J.N.
    Structural and functional characterisation of the methionine adenosyltransferase from Thermococcus kodakarensis (2013), BMC Struct. Biol., 13, 22.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 expressed in Escherichia coli BL21(DE3) cells Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.6 sitting drop vapor diffusion method, using 22% (w/v) polyethylene glycol 3350 and 0.2 M sodium citrate Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.31
-
 L-methionine at pH 8.0 and 37°C Thermococcus kodakarensis
2.5.1.6 6.54
-
 ATP at pH 8.0 and 37°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 44300
-
-
 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O Thermococcus kodakarensis
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 nickel affinity column chromatography Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Thermococcus kodakarensis phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 homodimer 2 * 40000-55000, SDS-PAGE Thermococcus kodakarensis
2.5.1.6 homodimer 2 * 44300, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.6 MAT
-
 Thermococcus kodakarensis
2.5.1.6 methionine adenosyltransferase
-
 Thermococcus kodakarensis
2.5.1.6 MetK
-
 Thermococcus kodakarensis
2.5.1.6 S-adenosylmethionine synthase
-
 Thermococcus kodakarensis