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Literature summary extracted from

  • Kang, L.; Bai, Y.; Cai, Y.; Zheng, X.
    Discovery of novel feruloyl esterase activity of BioH in Escherichia coli BL21(DE3) (2016), Biotechnol. Lett., 38, 1009-1013.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.5
-
ferulic acid ethyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 1.8
-
ferulic acid methyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 3.1
-
p-coumaric acid methyl ester pH 8.8, 40°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Escherichia coli
-
cf. EC 3.1.1.85
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ferulic acid ethyl ester + H2O
-
Escherichia coli ferulic acid + ethanol
-
?
3.1.1.73 ferulic acid methyl ester + H2O
-
Escherichia coli ferulic acid + methanol
-
?
3.1.1.73 additional information enzyme additionally acts as pimeloyl-[acyl-carrier protein] methyl ester esterase, reaction of EC 3.1.1.85, and to cleave esters such as vinyl laurate, propyl acetate, 1-naphthyl acetate, methyl alpha-bromophenylacetate Escherichia coli ?
-
?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
Escherichia coli p-coumaric acid + methanol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 BioH
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.9
-
ferulic acid ethyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 1.5
-
ferulic acid methyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 8.1
-
p-coumaric acid methyl ester pH 8.8, 40°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 0.9
-
ferulic acid methyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 2
-
ferulic acid ethyl ester pH 8.8, 40°C Escherichia coli
3.1.1.73 2.7
-
p-coumaric acid methyl ester pH 8.8, 40°C Escherichia coli