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Literature summary extracted from

  • Bouraoui, H.; Desrousseaux, M.L.; Ioannou, E.; Alvira, P.; Manai, M.; Remond, C.; Dumon, C.; Fernandez-Fuentes, N.; ODonohue, M.J.
    The GH51 alpha-L-arabinofuranosidase from Paenibacillus sp. THS1 is multifunctional, hydrolyzing main-chain and side-chain glycosidic bonds in heteroxylans (2016), Biotechnol. Biofuels, 9, 140.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 E296A about 1600fold decrease in activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.31
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 8.6
-
 4-nitrophenyl beta-D-galactofuranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 23.8
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.5, 60°C Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Paenibacillus sp. AM283040
-
-
3.2.1.55 Paenibacillus sp. THS1 AM283040
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 10.8
-
 substrate birchwood 4-O-methylglucuronoxylan, pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 14.4
-
 substrate beechwood 4-O-methylglucuronoxylan, pH 6.5, 60°C Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 4-nitrophenyl + H2O
-
 Paenibacillus sp. THS1 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. THS1 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-galactofuranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-galactofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-galactofuranoside + H2O
-
 Paenibacillus sp. THS1 4-nitrophenol + beta-D-galactofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-xylopyranoside
-
 Paenibacillus sp. 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-xylopyranoside
-
 Paenibacillus sp. THS1 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.55 beechwood 4-O-methylglucuronoxylan + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.55 birchwood 4-O-methylglucuronoxylan + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.55 additional information the enzyme additionally displays endo-xylanase activity, cleaving beta-1,4 bonds in heteroxylans Paenibacillus sp. ?
-
 ?
3.2.1.55 additional information the enzyme additionally displays endo-xylanase activity, cleaving beta-1,4 bonds in heteroxylans Paenibacillus sp. THS1 ?
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 75
-
 active up to Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 60
-
 stable up to Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 10.6
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 327
-
 4-nitrophenyl beta-D-galactofuranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 328
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.5, 60°C Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 7
-
 Paenibacillus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 0.44
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 38
-
 4-nitrophenyl beta-D-galactofuranoside pH 6.5, 60°C Paenibacillus sp.
3.2.1.55 1050
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.5, 60°C Paenibacillus sp.