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Literature summary extracted from

  • Wei, H.; Wang, W.; Alahuhta, M.; Vander, Wall, T.; Baker, J.O.; Taylor, L.E. 2nd; Decker, S.R.; Himmel, M.E.; Zhang, M.
    Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing (2014), Biotechnol. Biofuels, 7, 148.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.176 biofuel production successful expression of a chimeric cellobiohydrolase I with essentially full native activity in Yarrowia lipolytica. Yarrowia lipolytica strains can be genetically engineered, ultimately by heterologous expression of fungal cellulases and other enzymes, to directly convert lignocellulosic substrates to biofuels Trichoderma reesei
3.2.1.176 biofuel production successful expression of a chimeric cellobiohydrolase I with essentially full native activity in Yarrowia lipolytica. Yarrowia lipolytica strains can be genetically engineered, ultimately by heterologous expression of fungal cellulases and other enzymes, to directly convert lignocellulosic substrates to biofuels Rasamsonia emersonii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 folding and/or post-translation modification of heterologous enzyme in Yarrowia lipolytica cannot completely mimic that in the original source strain. Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei Trichoderma reesei
3.2.1.176 folding and/or post-translation modification of heterologous enzyme in Yarrowia lipolytica cannot completely mimic that in the original source strain. Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei Rasamsonia emersonii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 additional information expression of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei in Yarrowia lipolytica Trichoderma reesei
3.2.1.176 additional information expression of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei in Yarrowia lipolytica Rasamsonia emersonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.176 52000
-
 native enzyme produced in Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Trichoderma reesei
3.2.1.176 53000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Trichoderma reesei
3.2.1.176 53000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Rasamsonia emersonii
3.2.1.176 61000
-
 native enzyme produced in Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Trichoderma reesei
3.2.1.176 63000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Trichoderma reesei
3.2.1.176 63000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Rasamsonia emersonii Q8TFL9
-
-
3.2.1.176 Trichoderma reesei P62694 i.e. Hypocrea jecorina
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.176 glycoprotein
-
 Rasamsonia emersonii
3.2.1.176 glycoprotein in the case of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei which is expressed from Yarrowia, the overall magnitude of glycosylation is similar or only slightly higher than that for native cellobiohydrolase I produced in Trichoderma reesei Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 avicel + H2O
-
 Trichoderma reesei cellobiose + D-glucose
-
 ?
3.2.1.176 avicel + H2O
-
 Rasamsonia emersonii cellobiose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 cellobiohydrolase I
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase I
-
 Rasamsonia emersonii