Literature summary extracted from
Wei, R.; Oeser, T.; Schmidt, J.; Meier, R.; Barth, M.; Then, J.; Zimmermann, W.
Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition (2016), Biotechnol. Bioeng., 113, 1658-1665.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.1.101 |
degradation |
exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme |
Thermobifida fusca |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.1.101 |
G62A |
exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme. Mutant G62A reveals a 5.5fold lower binding constant to the inhibitor mono-(2-hydroxyethyl) terephthalate than the wild type enzyme |
Thermobifida fusca |
3.1.1.101 |
G62A/I213S |
exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme |
Thermobifida fusca |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.101 |
mono-(2-hydroxyethyl) terephthalate |
strong inhibition |
Thermobifida fusca |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.101 |
Thermobifida fusca |
E5BBQ3 |
cf. EC 3.1.1.74 |
- |
3.1.1.101 |
Thermobifida fusca KW3 |
E5BBQ3 |
cf. EC 3.1.1.74 |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.101 |
(ethylene terephthalate)n + H2O |
substrates are amorphous PET film or PET fibers |
Thermobifida fusca |
(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate |
terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products |
? |
|
3.1.1.101 |
(ethylene terephthalate)n + H2O |
substrates are amorphous PET film or PET fibers |
Thermobifida fusca KW3 |
(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate |
terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products |
? |
|