Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Batten, L.E.; Parnell, A.E.; Wells, N.J.; Murch, A.L.; Oyston, P.C.; Roach, P.L.
    Biochemical and structural characterization of polyphosphate kinase 2 from the intracellular pathogen Francisella tularensis (2016), Biosci. Rep., 36, e00294.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.4.1 drug development the enzym eis a target for inhibitor development Francisella tularensis subsp. tularensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 gene FTT1564, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta pLysS (DE3) Francisella tularensis subsp. tularensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.1 purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.23 A resolution, molecular replacement using the Sinorhizobium meliloti PPK2 structure (SMc02148, PDB ID 3CZQ) as a model Francisella tularensis subsp. tularensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 additional information construction of a DELTA FTT1564 enzyme gene deletion mutant strain Francisella tularensis subsp. tularensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 additional information
-
 additional information Michalis-Menten steady state kinetic analysis of FtPPK2 substrate specificity, overview Francisella tularensis subsp. tularensis
2.7.4.1 0.372
-
 ATP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 0.546
-
 ADP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 0.692
-
 GTP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 0.727
-
 GDP pH 8.0, 37°C Francisella tularensis subsp. tularensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ required, best at 10 mM Francisella tularensis subsp. tularensis
2.7.4.1 Mn2+ required, best at 1 mM Francisella tularensis subsp. tularensis
2.7.4.1 additional information lower optimal concentration for Mn2+ ions than Mg2+ ions Francisella tularensis subsp. tularensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ADP + (phosphate)n Francisella tularensis subsp. tularensis
-
 ATP + (phosphate)n-1
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Francisella tularensis subsp. tularensis Q5NEQ5 gene FTT1564
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta pLysS (DE3) by nickel affinity chromatography and gel filtration Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n
-
 Francisella tularensis subsp. tularensis ATP + (phosphate)n-1
-
 r
2.7.4.1 ATP + (phosphate)n+1
-
 Francisella tularensis subsp. tularensis ADP + (phosphate)n
-
 r
2.7.4.1 GDP + (phosphate)n
-
 Francisella tularensis subsp. tularensis GTP + (phosphate)n-1
-
 r
2.7.4.1 GTP + (phosphate)n+1
-
 Francisella tularensis subsp. tularensis GDP + (phosphate)n
-
 r
2.7.4.1 additional information the substrate specificity of FtPPK2 includes purine but not pyrimidine nucleotides. No activity with UDP and CDP as substrates. No formation of ADP or GDP from AMP or GMP Francisella tularensis subsp. tularensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 More the structure consists of a six-stranded parallel beta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family and the thymidylate kinase superfamily Francisella tularensis subsp. tularensis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyphosphate kinase 2
-
 Francisella tularensis subsp. tularensis
2.7.4.1 PPK2
-
 Francisella tularensis subsp. tularensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 37
-
 assay at Francisella tularensis subsp. tularensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 1.46
-
 ATP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 2.77
-
 GTP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 3.17
-
 ADP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 3.69
-
 GDP pH 8.0, 37°C Francisella tularensis subsp. tularensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 8
-
 assay at Francisella tularensis subsp. tularensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 additional information
-
 maximal activity at pH 8.0, sharp drop-off in activity at pH 9 Francisella tularensis subsp. tularensis

General Information

EC Number General Information Comment Organism
2.7.4.1 evolution a polyphosphate kinase from the polyphosphate kinase 2 (PPK2) family. The enzyme structure consists of a six-stranded parallelbeta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family and the thymidylate kinase superfamily Francisella tularensis subsp. tularensis
2.7.4.1 malfunction the isogenic deletion mutant is defective for intracellular growth in macrophages and is attenuated in mice. The DELTAFTT1564 strain shows significantly increased sensitivity to a range of antibiotics in a manner independent of the mode of action of the antibiotic Francisella tularensis subsp. tularensis
2.7.4.1 additional information a lid-loop and the conserved Walker A and B motifs are important for substrate binding and enzyme catalysis Francisella tularensis subsp. tularensis
2.7.4.1 physiological function important role for polyphosphate in the virulence of Francisella Francisella tularensis subsp. tularensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.1 3.92
-
 ATP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 4.002
-
 GTP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 5.075
-
 GDP pH 8.0, 37°C Francisella tularensis subsp. tularensis
2.7.4.1 5.788
-
 ADP pH 8.0, 37°C Francisella tularensis subsp. tularensis