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Literature summary extracted from

  • Wilkinson, R.C.; Batten, L.E.; Wells, N.J.; Oyston, P.C.; Roach, P.L.
    Biochemical studies on Francisella tularensis RelA in (p)ppGpp biosynthesis (2015), Biosci. Rep., 35, e00268.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.5 guanosine-5',3'-dibisphosphate ppGpp, potential allosteric regulator, activates the enzyme with an EC50 of 0.060 mM Francisella tularensis subsp. tularensis
2.7.6.5 additional information activation of Francisella tularensis RelA by stalled ribosomal complexes formed with ribosomes purified from Escherichia coli MRE600, and significantly weaker activation with ribosomes isolated from Francisella philomiragia Francisella tularensis subsp. tularensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.5 gene relA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS Francisella tularensis subsp. tularensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.5 additional information
-
 additional information steady-state kinetic analysis, saturation activity curves best fit a sigmoidal function, the kinetic profile can result from allosteric regulation, overview Francisella tularensis subsp. tularensis
2.7.6.5 0.259 0.344 ATP pH 8.0, 30°C, recombinant enzyme Francisella tularensis subsp. tularensis
2.7.6.5 0.801 1.024 GTP pH 8.0, 30°C, recombinant enzyme Francisella tularensis subsp. tularensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.5 Mg2+ required Francisella tularensis subsp. tularensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.5 74000
-
-
 Francisella tularensis subsp. tularensis
2.7.6.5 128000
-
 recombinant His-tagged enzyme, gel filtration Francisella tularensis subsp. tularensis
2.7.6.5 148000
-
 about, sequence calculation Francisella tularensis subsp. tularensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.5 ATP + GTP Francisella tularensis subsp. tularensis
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.5 Francisella tularensis subsp. tularensis Q5NEV0 gene relA or FTT_1508c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.5 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and gel filtration Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.5 ATP + GTP
-
 Francisella tularensis subsp. tularensis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 ATP + GTP high degree of specificity for GTP as a pyrophosphate acceptor, with no measurable turnover for GDP Francisella tularensis subsp. tularensis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.5 homodimer 2 * 74000, recombinant His-tagged enzyme, SDS-PAGE Francisella tularensis subsp. tularensis

Synonyms

EC Number Synonyms Comment Organism
2.7.6.5 RelA
-
 Francisella tularensis subsp. tularensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.5 30
-
 assay at Francisella tularensis subsp. tularensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.5 8
-
 assay at Francisella tularensis subsp. tularensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.6.5 ATP
-
 Francisella tularensis subsp. tularensis

General Information

EC Number General Information Comment Organism
2.7.6.5 evolution the enzyme RelA belongs to the RSH superfamily, also comprising members such as RelA/SpoT homologue, (p)ppGpp synthetase I, SpoT, and (p)ppGpp synthetase II. The RSH enzyme family controls concentrations of the alarmones (p)ppGpp (guanosine penta- or tetra-phosphate) Francisella tularensis subsp. tularensis