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Literature summary extracted from

  • Kamada, S.; Okugochi, T.; Asano, K.; Tobe, R.; Mihara, H.; Nemoto, M.; Inagaki, K.; Tamura, T.
    A non-radioactive assay for selenophosphate synthetase activity using recombinant pyruvate pyrophosphate dikinase from Thermus thermophilus HB8 (2016), Biosci. Biotechnol. Biochem., 80, 1970-1972.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 gene Sephs2, recombinant expression of C-terminally His6-tagged enzyme mutant Sephs2U60C in Escherichia coli Rosetta (DE3) pLysS Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 Sec60C site-directed mutagenesis, the in-frame opal codon for Sec60 is altered to Cys-coding TGT in mutant Sephs2U60C, the substitution of active site Sec with Cys does not significantly perturb the catalytic function of the His6-tagged human Sephs2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.3 additional information
-
 additional information steady-state kinetics for the C-terminally His6-tagged enzyme mutant Sephs2-U60C Homo sapiens
2.7.9.3 0.026
-
 ATP pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.3 ATP + selenide + H2O Homo sapiens
-
 AMP + selenophosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Homo sapiens Q99611 gene Sephs2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant C-terminally His6-tagged enzyme mutant Sephs2U60C from Escherichia coli Rosetta (DE3) pLysS by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O
-
 Homo sapiens AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 additional information development of a non-radioactive assay for detection of selenophosphate synthetase activity using recombinant pyruvate diphosphate dikinase, PPDK, from Thermus thermophilus strain HB8 in an enzyme-coupled reaction system, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.9.3 Sephs2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.9.3 0.0058
-
 ATP pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.3 ATP
-
 Homo sapiens