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Literature summary extracted from

  • Takeda, M.; Matsumura, A.T.; Kurosaki, K.; Chhetri, R.T.; Motomatsu, S.; Suzuki, I.; Sahabi, D.M.
    An enantioselective NADP(+)-dependent alcohol dehydrogenase responsible for cooxidative production of (3S)-5-hydroxy-3-methyl-pentanoic acid (2016), Biosci. Biotechnol. Biochem., 80, 1155-1163.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 Cu2+ 1 mM, complete loss of activity Mycobacterium sp.
1.1.1.2 iodoacetic acid 1 mM, 72.6% residual activity Mycobacterium sp.
1.1.1.2 NaN3 1 mM, complete loss of activity Mycobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 10.27
-
 3-methyl-1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 23.38
-
 1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 26.88
-
 NADP+ pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 43.75
-
 butan-1-ol pH 7.0. 30°C Mycobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.2 soluble
-
 Mycobacterium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.2 Zn2+ 1 mM, 467% of initial activity Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 38000
-
-
 Mycobacterium sp.
1.1.1.2 68000
-
 gel filtration Mycobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Mycobacterium sp. A0A125SUY5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1,5-pentanediol + NADP+
-
 Mycobacterium sp. ? + NADPH + H+
-
 ?
1.1.1.2 3-methyl-1,5-pentanediol + NADP+
-
 Mycobacterium sp. ?
-
 ?
1.1.1.2 butan-1-ol + NADP+
-
 Mycobacterium sp. butanal + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer 2 * 38000, SDS-PAGE, 2 * 36907, calculated Mycobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 Mpd1
-
 Mycobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 45
-
-
 Mycobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 45
-
 1 h, complete inactivation above 45°C Mycobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 5.31
-
 1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 7.02
-
 butan-1-ol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 7.84
-
 3-methyl-1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 13.41
-
 NADP+ pH 7.0. 30°C Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7 7.5
-
 Mycobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 additional information no cofoactor: NADH Mycobacterium sp.
1.1.1.2 NADPH
-
 Mycobacterium sp.

General Information

EC Number General Information Comment Organism
1.1.1.2 physiological function bacterium Mycobacterium sp. B-009, is able to grow on racemic 1,2-propanediol. The strain oxidizes 3-methyl-1,5-pentanediol to 5-hydroxy-3-methyl-pentanoic acid via the S-form of 5-hydroxy-3-methyl-pentanoic acid. The enzyme is an enantioselective 3-methyl-1,5-pentanediol dehydrogenase Mycobacterium sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 160
-
 butan-1-ol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 230
-
 1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 500
-
 NADP+ pH 7.0. 30°C Mycobacterium sp.
1.1.1.2 760
-
 3-methyl-1,5-pentanediol pH 7.0. 30°C Mycobacterium sp.