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Literature summary extracted from

  • Urabe, H.; Ogawara, H.; Motojima, K.
    Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE (2015), Biosci. Biotechnol. Biochem., 79, 855-862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 cytoplasm
-
 Streptomyces coelicolor 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 55600
-
-
 Streptomyces coelicolor
2.7.11.1 80000
-
-
 Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + KbpA protein Streptomyces coelicolor
-
 ADP + phosphorylated KbpA protein
-
 ?
2.7.11.1 ATP + KbpA protein Streptomyces coelicolor A3 (2) M145
-
 ADP + phosphorylated KbpA protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Streptomyces coelicolor Q9ZNB6
-
-
2.7.11.1 Streptomyces coelicolor A3 (2) M145 Q9ZNB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 glutathione Sepharose column chromatography, and gel filtration Streptomyces coelicolor

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.11.1 the recombinant enzyme is refolded into active forms by stepwise dialysis against buffer containing 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 1 mM dithiothreitol with decreasing urea concentration of 4, 2, and 1 M at 4 °C for 1 h Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + KbpA protein
-
 Streptomyces coelicolor ADP + phosphorylated KbpA protein
-
 ?
2.7.11.1 ATP + KbpA protein
-
 Streptomyces coelicolor A3 (2) M145 ADP + phosphorylated KbpA protein
-
 ?
2.7.11.1 additional information the recombinant enzyme is autophosphorylated at threonine residues only Streptomyces coelicolor ?
-
 ?
2.7.11.1 additional information the recombinant enzyme is autophosphorylated at threonine residues only Streptomyces coelicolor A3 (2) M145 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 ? x * 55600, calculated from amino acid sequence Streptomyces coelicolor
2.7.11.1 ? x * 80000, GST-tagged enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PkaE
-
 Streptomyces coelicolor
2.7.11.1 serine/threonine protein kinase
-
 Streptomyces coelicolor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.1 Streptomyces coelicolor calculated from amino acid sequence
-
 6.2

General Information

EC Number General Information Comment Organism
2.7.11.1 physiological function the enzyme acts as a negative regulator for blue pigment production Streptomyces coelicolor