EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 0.02 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 0.09 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 0.48 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 5.65 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.76 | 42.2 | - |
(R,S)-acetoin | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 0.05 | - |
NADH | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 4.47 | - |
diacetyl | pH 7.0, 30°C | Mycobacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 36000 | - |
- |
Mycobacterium sp. |
1.1.1.1 | 150000 | - |
gel filtration | Mycobacterium sp. |
1.1.1.76 | 36000 | - |
- |
Mycobacterium sp. |
1.1.1.76 | 150000 | - |
gel filtration | Mycobacterium sp. |
1.1.1.304 | 36000 | - |
- |
Mycobacterium sp. |
1.1.1.304 | 150000 | - |
gel filtration | Mycobacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.1 | Mycobacterium sp. | W8VSK8 | - |
- |
1.1.1.76 | Mycobacterium sp. | W8VSK8 | - |
- |
1.1.1.304 | Mycobacterium sp. | W8VSK8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.1 | 1,5-pentanediol + NAD+ | 18% of the activity with butan-1-ol | Mycobacterium sp. | ? + NADH + H+ | - |
? | |
1.1.1.1 | butan-1-ol + NAD+ | best substrate | Mycobacterium sp. | butanal + NADH + H+ | - |
r | |
1.1.1.1 | butanal + NADH + H+ | - |
Mycobacterium sp. | butan-1-ol + NAD+ | - |
r | |
1.1.1.1 | ethanol + NAD+ | 12% of the activity with butan-1-ol | Mycobacterium sp. | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.1 | additional information | enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities | Mycobacterium sp. | ? | - |
? | |
1.1.1.1 | propan-1-ol + NAD+ | 55% of the activity with butan-1-ol | Mycobacterium sp. | propanal + NADH + H+ | - |
r | |
1.1.1.1 | propan-2-ol + NAD+ | 65% of the activity with butan-1-ol | Mycobacterium sp. | acetone + NADH + H+ | - |
r | |
1.1.1.76 | (2S,3S)-2,3-butanediol + NAD+ | - |
Mycobacterium sp. | (R,S)-acetoin + NADH + H+ | selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol | r | |
1.1.1.76 | (R,S)-acetoin + NADH + H+ | - |
Mycobacterium sp. | (2S,3S)-2,3-butanediol + meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.76 | meso-2,3-butanediol + NAD+ | - |
Mycobacterium sp. | (R,S)-acetoin + NADH + H+ | selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol | r | |
1.1.1.76 | additional information | enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities | Mycobacterium sp. | ? | - |
? | |
1.1.1.304 | diacetyl + NADH + H+ | - |
Mycobacterium sp. | (S)-acetoin + NAD+ | - |
? | |
1.1.1.304 | additional information | enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities | Mycobacterium sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.1 | tetramer | 4 * 36000, SDS-PAGE, 4 * 35971, calculated | Mycobacterium sp. |
1.1.1.76 | tetramer | 4 * 36000, SDS-PAGE, 4 * 35971, calculated | Mycobacterium sp. |
1.1.1.304 | tetramer | 4 * 36000, SDS-PAGE, 4 * 35971, calculated | Mycobacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.1 | ADS1 | - |
Mycobacterium sp. |
1.1.1.76 | ADS1 | - |
Mycobacterium sp. |
1.1.1.304 | ADS1 | - |
Mycobacterium sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 1 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 2 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 102 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 153 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.76 | 6 | - |
(R,S)-acetoin | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 110 | - |
NADH | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 163 | - |
diacetyl | pH 7.0, 30°C | Mycobacterium sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.1 | Mycobacterium sp. | isoelectric focusing | - |
4.8 |
1.1.1.1 | Mycobacterium sp. | calculated | - |
5.1 |
1.1.1.76 | Mycobacterium sp. | isoelectric focusing | - |
4.8 |
1.1.1.76 | Mycobacterium sp. | calculated | - |
5.1 |
1.1.1.304 | Mycobacterium sp. | isoelectric focusing | - |
4.8 |
1.1.1.304 | Mycobacterium sp. | calculated | - |
5.1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 0.3 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 80 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 210 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.1 | 2000 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.76 | 0.1 | - |
(R,S)-acetoin | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 36.4 | - |
diacetyl | pH 7.0, 30°C | Mycobacterium sp. | |
1.1.1.304 | 210 | - |
NADH | pH 7.0, 30°C | Mycobacterium sp. |