Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nieter, A.; Kelle, S.; Linke, D.; Berger, R.
    Feruloyl esterases from Schizophyllum commune to treat food industry side-streams (2016), Biores. Technol., 220, 38-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.044
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.059
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.114
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.123
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.124
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.131
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.137
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.147
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.151
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 0.16
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Schizophyllum commune D8QHC3
-
-
3.1.1.73 Schizophyllum commune D8QHD6
-
-
3.1.1.73 Schizophyllum commune H4-8 D8QHC3
-
-
3.1.1.73 Schizophyllum commune H4-8 D8QHD6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-trans-feruloyl-L-arabinofuranose + H2O 88% of the activity with methyl p-coumarate Schizophyllum commune L-arabinofuranose + ferulate
-
 ?
3.1.1.73 5-O-trans-feruloyl-L-arabinofuranose + H2O 90% of the activity with methyl p-coumarate Schizophyllum commune L-arabinofuranose + ferulate
-
 ?
3.1.1.73 alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O 93% of the activity with methyl p-coumarate Schizophyllum commune alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
 ?
3.1.1.73 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O 96% of the activity with methyl p-coumarate Schizophyllum commune beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
 ?
3.1.1.73 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O 97% of the activity with methyl p-coumarate Schizophyllum commune beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
 ?
3.1.1.73 chlorogenic acid + H2O 11% of the activity with methyl p-coumarate Schizophyllum commune caffeate + quinate
-
 ?
3.1.1.73 chlorogenic acid + H2O 11% of the activity with methyl p-coumarate Schizophyllum commune H4-8 caffeate + quinate
-
 ?
3.1.1.73 coffee pulp + H2O
-
 Schizophyllum commune ferulic acid + caffeic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-5-O-trans-feruloyl-L-arabinofuranose + H2O 94% of the activity with methyl p-coumarate Schizophyllum commune D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-L-arabinofuranose + ferulate
-
 ?
3.1.1.73 D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-5-O-trans-feruloyl-L-arabinofuranose + H2O 95% of the activity with methyl p-coumarate Schizophyllum commune D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-L-arabinofuranose + ferulate
-
 ?
3.1.1.73 destarched wheat bran + H2O
-
 Schizophyllum commune ferulic acid + ?
-
 ?
3.1.1.73 destarched wheat bran + H2O
-
 Schizophyllum commune H4-8 ferulic acid + ?
-
 ?
3.1.1.73 ethyl ferulate + H2O 62% of the activity with methyl p-coumarate Schizophyllum commune ethanol + ferulate
-
 ?
3.1.1.73 ethyl ferulate + H2O 70% of the activity with methyl p-coumarate Schizophyllum commune ethanol + ferulate
-
 ?
3.1.1.73 methyl 4-hydroxybenzoate + H2O 27% of the activity with methyl p-coumarate Schizophyllum commune methanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 methyl 4-hydroxybenzoate + H2O 31% of the activity with methyl p-coumarate Schizophyllum commune methanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 methyl 4-hydroxybenzoate + H2O 27% of the activity with methyl p-coumarate Schizophyllum commune H4-8 methanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 methyl 4-hydroxybenzoate + H2O 31% of the activity with methyl p-coumarate Schizophyllum commune H4-8 methanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 methyl caffeate + H2O 47% of the activity with methyl p-coumarate Schizophyllum commune methanol + caffeate
-
 ?
3.1.1.73 methyl caffeate + H2O 50% of the activity with methyl p-coumarate Schizophyllum commune methanol + caffeate
-
 ?
3.1.1.73 methyl ferulate + H2O 65% of the activity with methyl p-coumarate Schizophyllum commune methanol + ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O 72% of the activity with methyl p-coumarate Schizophyllum commune methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Schizophyllum commune methanol + p-coumarate
-
 ?
3.1.1.73 additional information enzyme preferably hydrolyzes feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and shows an ability to hydrolyze benzoic acid esters Schizophyllum commune ?
-
 ?
3.1.1.73 additional information enzyme preferably hydrolyzes feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and shows an ability to hydrolyze benzoic acid esters Schizophyllum commune H4-8 ?
-
 ?
3.1.1.73 sugar beet pectin + H2O
-
 Schizophyllum commune ferulic acid + ?
-
 ?
3.1.1.73 sugar beet pectin + H2O
-
 Schizophyllum commune H4-8 ferulic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FaeD1
-
 Schizophyllum commune
3.1.1.73 FaeD2
-
 Schizophyllum commune
3.1.1.73 SCHCODRAFT_60993
-
 Schizophyllum commune
3.1.1.73 SCHCODRAFT_61055
-
 Schizophyllum commune

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 6.6
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 7.5
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 11.2
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 12.5
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 34.1
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 38.4
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 51
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 54.7
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 91.4
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 105.1
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 57.3
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 58.2
-
 chlorogenic acid pH 7.5, 37°C Schizophyllum commune
3.1.1.73 210
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 257.2
-
 methyl 4-hydroxybenzoate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 275.1
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 310.6
-
 methyl caffeate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 319.2
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 372.6
-
 methyl ferulate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 666.2
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune
3.1.1.73 696.3
-
 methyl p-coumarate pH 7.5, 37°C Schizophyllum commune