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Literature summary extracted from

  • Boehme, C.; Bieber, F.; Linnemann, J.; Breitling, R.; Lorkowski, S.; Reissmann, S.
    Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate kinase (2013), Biol. Chem., 394, 695-701.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expressed in Escherichia coli BL21(DE3) cells Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 Co2+
-
Oryctolagus cuniculus
2.7.1.40 Cu2+
-
Oryctolagus cuniculus
2.7.1.40 Mn2+
-
Oryctolagus cuniculus
2.7.1.40 Ni2+
-
Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.073
-
phosphoenolpyruvate recombinant enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
2.7.1.40 0.076
-
phosphoenolpyruvate native enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
2.7.1.40 0.357
-
ADP native enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
2.7.1.40 0.365
-
ADP recombinant enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ necessary for full enzymatic activity Oryctolagus cuniculus
2.7.1.40 Mg2+ necessary for full enzymatic activity Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 58000
-
recombinant enzyme, gel filtration Oryctolagus cuniculus
2.7.1.40 59000
-
native enzyme, gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate Oryctolagus cuniculus
-
ATP + pyruvate
-
?
2.7.1.40 TDP + phosphoenolpyruvate Oryctolagus cuniculus
-
TTP + pyruvate 95% yield ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 Ni-IDA affinity column chromatography, and gel filtration Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 muscle
-
Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 -20°C, with 50% (v/v) glycerol, 72 h, no loss of activity Oryctolagus cuniculus
2.7.1.40 30°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 70% loss of activity Oryctolagus cuniculus
2.7.1.40 37°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 95% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
Oryctolagus cuniculus ATP + pyruvate
-
?
2.7.1.40 additional information the recombinant enzyme shows the following range in its substrate specificity: ADP>dADP>dGDP>dCDP>TDP Oryctolagus cuniculus ?
-
?
2.7.1.40 TDP + phosphoenolpyruvate
-
Oryctolagus cuniculus TTP + pyruvate 95% yield ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 homodimer 2 * 29495, MALDI-TOF spectrometry Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 M1-PK
-
Oryctolagus cuniculus
2.7.1.40 pyruvate phosphotransferase
-
Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 37
-
-
Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7.4 7.8
-
Oryctolagus cuniculus