EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.40 | expressed in Escherichia coli BL21(DE3) cells | Oryctolagus cuniculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | Co2+ | - |
Oryctolagus cuniculus | |
2.7.1.40 | Cu2+ | - |
Oryctolagus cuniculus | |
2.7.1.40 | Mn2+ | - |
Oryctolagus cuniculus | |
2.7.1.40 | Ni2+ | - |
Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.40 | 0.073 | - |
phosphoenolpyruvate | recombinant enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
2.7.1.40 | 0.076 | - |
phosphoenolpyruvate | native enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
2.7.1.40 | 0.357 | - |
ADP | native enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
2.7.1.40 | 0.365 | - |
ADP | recombinant enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | K+ | necessary for full enzymatic activity | Oryctolagus cuniculus | |
2.7.1.40 | Mg2+ | necessary for full enzymatic activity | Oryctolagus cuniculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | 58000 | - |
recombinant enzyme, gel filtration | Oryctolagus cuniculus |
2.7.1.40 | 59000 | - |
native enzyme, gel filtration | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.40 | ADP + phosphoenolpyruvate | Oryctolagus cuniculus | - |
ATP + pyruvate | - |
? | |
2.7.1.40 | TDP + phosphoenolpyruvate | Oryctolagus cuniculus | - |
TTP + pyruvate | 95% yield | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.40 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.40 | Ni-IDA affinity column chromatography, and gel filtration | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.40 | muscle | - |
Oryctolagus cuniculus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.40 | -20°C, with 50% (v/v) glycerol, 72 h, no loss of activity | Oryctolagus cuniculus |
2.7.1.40 | 30°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 70% loss of activity | Oryctolagus cuniculus |
2.7.1.40 | 37°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 95% loss of activity | Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.40 | ADP + phosphoenolpyruvate | - |
Oryctolagus cuniculus | ATP + pyruvate | - |
? | |
2.7.1.40 | additional information | the recombinant enzyme shows the following range in its substrate specificity: ADP>dADP>dGDP>dCDP>TDP | Oryctolagus cuniculus | ? | - |
? | |
2.7.1.40 | TDP + phosphoenolpyruvate | - |
Oryctolagus cuniculus | TTP + pyruvate | 95% yield | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.40 | homodimer | 2 * 29495, MALDI-TOF spectrometry | Oryctolagus cuniculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.40 | M1-PK | - |
Oryctolagus cuniculus |
2.7.1.40 | pyruvate phosphotransferase | - |
Oryctolagus cuniculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | 37 | - |
- |
Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.40 | 7.4 | 7.8 | - |
Oryctolagus cuniculus |