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Literature summary extracted from

  • Meux, E.; Morel, M.; Lamant, T.; Gérardin, P.; Jacquot, J.; Dumarcay, S.; Gelhaye, E.
    New substrates and activity of Phanerochaete chrysosporium Omega glutathione transferases (2013), Biochimie, 95, 336-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 C18S the mutant of isoform GSTO4 exhibits a similar pattern of activity than the wild type protein Phanerodontia chrysosporium
2.5.1.18 C18S the mutant of isoform GSTO7 exhibits a similar pattern of activity than the wild type protein Phanerodontia chrysosporium
2.5.1.18 C37S inactive mutant of isoform GSTO3 Phanerodontia chrysosporium
2.5.1.18 C37S inactive mutant of isoform GSTO4 Phanerodontia chrysosporium
2.5.1.18 C91S the mutant of isoform GSTO4 exhibits a similar pattern of activity than the wild type protein Phanerodontia chrysosporium
2.5.1.18 C91S the mutant of isoform GSTO7 exhibits a similar pattern of activity than the wild type protein Phanerodontia chrysosporium
2.5.1.18 S15C the inactive mutant of isoform GSTO4 is able to bind covalently a glutathione Phanerodontia chrysosporium
2.5.1.18 S15C the inactive mutant of isoform GSTO7 is able to bind covalently a glutathione Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 2-bromo-1-tetralone
-
 Phanerodontia chrysosporium
2.5.1.18 camphene
-
 Phanerodontia chrysosporium
2.5.1.18 carene
-
 Phanerodontia chrysosporium
2.5.1.18 caryophyllene
-
 Phanerodontia chrysosporium
2.5.1.18 caryophyllene oxide
-
 Phanerodontia chrysosporium
2.5.1.18 gallic acid
-
 Phanerodontia chrysosporium
2.5.1.18 limonene
-
 Phanerodontia chrysosporium
2.5.1.18 longifolene
-
 Phanerodontia chrysosporium
2.5.1.18 menadione
-
 Phanerodontia chrysosporium
2.5.1.18 myrcene
-
 Phanerodontia chrysosporium
2.5.1.18 Pentachlorophenol
-
 Phanerodontia chrysosporium
2.5.1.18 phenylacetophenone
-
 Phanerodontia chrysosporium
2.5.1.18 pinene
-
 Phanerodontia chrysosporium
2.5.1.18 rifampicin
-
 Phanerodontia chrysosporium
2.5.1.18 S-glutathionyl-phenylacetophenone
-
 Phanerodontia chrysosporium
2.5.1.18 S-glutationyl-thiodione
-
 Phanerodontia chrysosporium
2.5.1.18 S-phenylacetylglutathione
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.14
-
 phenethyl isothiocyanate isoform GSTO7, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.207
-
 S-phenylacetylglutathione isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 1.222
-
 1-chloro-2,4-dinitrobenzene isoform GSTO7, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 1.66
-
 dehydroascorbate isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 26938
-
-
 Phanerodontia chrysosporium
2.5.1.18 30019
-
-
 Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Phanerodontia chrysosporium
-
 isoform GSTO4
-
2.5.1.18 Phanerodontia chrysosporium B3VQJ6 isoform GSTO3
-
2.5.1.18 Phanerodontia chrysosporium J7JYU5 isoform GSTO7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Phanerodontia chrysosporium S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 dehydroascorbate + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 additional information isoform GSTO7 shows no activity with hydroxyethyldisulfide, dehydroascorbate, and S-glutathionyl-phenylacetophenone Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 S-glutathionyl-phenylacetophenone + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 S-glutathionyltetralone + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 S-phenylacetylglutathione + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 26938, isoform GSTO7, calculated from amino acid sequence Phanerodontia chrysosporium
2.5.1.18 ? x * 26938, native isoform GSTO7, mass spectrometry Phanerodontia chrysosporium
2.5.1.18 ? x * 27713, isoform GSTO4, calculated from amino acid sequence Phanerodontia chrysosporium
2.5.1.18 ? x * 30019, native isoform GSTO4, mass spectrometry Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTO3 isoform Phanerodontia chrysosporium
2.5.1.18 GSTO4 isoform Phanerodontia chrysosporium
2.5.1.18 GSTO7 isoform Phanerodontia chrysosporium
2.5.1.18 omega glutathione transferase
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 1.26
-
 dehydroascorbate isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 1.69
-
 phenethyl isothiocyanate isoform GSTO7, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 2.08
-
 1-chloro-2,4-dinitrobenzene isoform GSTO7, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 180
-
 S-phenylacetylglutathione isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 0.00061
-
 S-glutathionyl-phenylacetophenone isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.00087
-
 S-phenylacetylglutathione isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.001691
-
 phenylacetophenone isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.003148
-
 S-glutationyl-thiodione isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.003168
-
 phenylacetophenone isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.004542
-
 S-glutationyl-thiodione isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.01444
-
 Pentachlorophenol isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.0161
-
 2-bromo-1-tetralone isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.01632
-
 2-bromo-1-tetralone isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.02111
-
 rifampicin isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.02498
-
 menadione isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.03591
-
 menadione isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.04558
-
 rifampicin isoform GSTO4, at pH 6.5 and 20°C Phanerodontia chrysosporium
2.5.1.18 0.06904
-
 Pentachlorophenol isoform GSTO3, at pH 6.5 and 20°C Phanerodontia chrysosporium