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Literature summary extracted from

  • Yoshida, Y.; Sato, M.; Nagano, K.; Hasegawa, Y.; Okamoto, T.; Yoshimura, F.
    Production of 4-hydroxybutyrate from succinate semialdehyde in butyrate biosynthesis in Porphyromonas gingivalis (2015), Biochim. Biophys. Acta, 1850, 2582-2591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.61 expressed in Escherichia coli BL21 cells Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.61 0.15
-
 succinate semialdehyde with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis
1.1.1.61 0.41
-
 NADH with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.61 Mn2+ most favorable metal ion for enzymatic activity Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.61 37000
-
 1 * 37000, SDS-PAGE Porphyromonas gingivalis
1.1.1.61 42000
-
 gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.61 succinate semialdehyde + NADH + H+ Porphyromonas gingivalis
-
 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.61 succinate semialdehyde + NADH + H+ Porphyromonas gingivalis ATCC 33277
-
 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.61 succinate semialdehyde + NADPH + H+ Porphyromonas gingivalis
-
 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.61 succinate semialdehyde + NADPH + H+ Porphyromonas gingivalis ATCC 33277
-
 4-hydroxybutanoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Porphyromonas gingivalis
-
-
-
1.1.1.61 Porphyromonas gingivalis ATCC 33277
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.61 glutathione Sepharose column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 succinate semialdehyde + NADH + H+
-
 Porphyromonas gingivalis 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.61 succinate semialdehyde + NADH + H+
-
 Porphyromonas gingivalis ATCC 33277 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.61 succinate semialdehyde + NADPH + H+
-
 Porphyromonas gingivalis 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.61 succinate semialdehyde + NADPH + H+
-
 Porphyromonas gingivalis ATCC 33277 4-hydroxybutanoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.61 monomer 1 * 37000, SDS-PAGE Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.61 PGN 0724 protein
-
 Porphyromonas gingivalis
1.1.1.61 succinate semialdehyde reductase
-
 Porphyromonas gingivalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.61 1329
-
 succinate semialdehyde with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.61 8
-
-
 Porphyromonas gingivalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.61 7 9 more than 80% activity between pH 7.0 and 9.0 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.61 NADH NADH is a more favorable cofactor for the enzyme than NADPH Porphyromonas gingivalis
1.1.1.61 NADPH NADH is a more favorable cofactor for the enzyme than NADPH Porphyromonas gingivalis